CAREER: "Holey Scaffold" Sensing System for Characterization of the Spatiotemporal Tumor Response to Nanoparticle-Mediated Photothermal and Photochemical Therapy

职业：“孔支架”传感系统，用于表征纳米粒子介导的光热和光化学疗法的时空肿瘤反应

基本信息

批准号：
1505412
负责人：
Marissa Rylander
金额：
$ 27.94万
依托单位：
University of Texas at Austin
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2014
资助国家：
美国
起止时间：
2014-08-10 至 2016-09-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1505412&HistoricalAwards=false
关键词：
CAREER Holey Scaffold Sensing System

项目摘要

0955072RylanderThe goal of this CAREER plan is to create and utilize a novel sensing system called the "holey scaffold" to nondestructively and minimally invasively measure dynamic nanoparticle transport and photothermal and photochemical tumor response to nanoparticle-mediated laser therapy. A holey scaffold is a new technology co-invented by the PI that is composed of an embedded network of microchannels within a scaffold framework upon which cells and tissues can be grown. Hollow core fibers integrated in the holey scaffold permit simultaneous optical sensing/imaging of dynamic processes and selective delivery of biological agents (e.g. nutrients, cells, nanoparticles) for control of biochemical responses. The CAREER objective is to use holey scaffolds to measure 1) spatiotemporal nanoparticle transport for varying nanoparticle properties, targeting approaches, and delivery methods, 2) dynamic photothermal response (temperature, heat shock protein expression, and cell injury), and 3) real-time photochemical response (reactive oxygen species production) associated with nanoparticle-mediated laser therapies. The versatility of holey scaffolds will enable the first measurement of dynamic tumor response in vitro within a bioreactor system and in vivo in a mouse tumor model. Measured tumor response will be utilized to create a novel computational model for predicting tumor response and optimizing implementation of nanoparticles in laser therapy.

0955072Rylander 这个职业计划的目标是创建和利用一种称为“多孔支架”的新型传感系统，以非破坏性和微创方式测量动态纳米颗粒传输以及对纳米颗粒介导的激光治疗的光热和光化学肿瘤反应。多孔支架是 PI 共同发明的一项新技术，由支架框架内嵌入的微通道网络组成，细胞和组织可以在支架框架上生长。集成在多孔支架中的空心纤维允许同时对动态过程进行光学传感/成像，并选择性地递送生物制剂（例如营养物、细胞、纳米颗粒）以控制生化反应。职业目标是使用多孔支架来测量 1) 不同纳米粒子特性的时空纳米粒子传输、靶向方法和递送方法，2) 动态光热响应（温度、热休克蛋白表达和细胞损伤），以及 3) 真实的光热反应。与纳米颗粒介导的激光疗法相关的时间光化学反应（活性氧物质的产生）。多孔支架的多功能性将能够首次测量生物反应器系统中的体外动态肿瘤反应和小鼠肿瘤模型的体内动态肿瘤反应。测量的肿瘤反应将用于创建一种新的计算模型，用于预测肿瘤反应并优化激光治疗中纳米颗粒的实施。