UNS: A functional nanocomposite-based biosensor for real-time ambulatory monitoring of salivary biomarkers

UNS：一种基于功能性纳米复合材料的生物传感器，用于实时动态监测唾液生物标志物

• 批准号: 1512816
• 负责人: Li-Jing Cheng
• 金额: $ 33.91万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1512816&HistoricalAwards=false
• 关键词: UNS; functional; nanocomposite; based; biosensor

1512816 Cheng, Li-Jing This research will develop a novel sensor device which will be able to measure molecules that are found in saliva when a person is under psychological stress. The study if successful will enable measurement of stress markers in ambulatory setting. Because the biosensor will be equipped with wireless connectivity, one can monitor stress experienced by the individual remotely. Real-time, detection of the stress salivary biomarkers enables assessment of stress levels in individuals. The salivary biomarkers are typically analyzed by fluorescence immunoassay and enzyme kinetic method which cannot be done in situ. The proposed stress biosensor built in a wearable configuration, will significantly benefit those regularly involved in stress-intensive activities, such as soldiers, pilots, and emergency care professionals. With the sensing function, stress and fatigue could be detected early in order to avoid impairment it may cause. Intellectual Merit: The proposed sensor consists of a composite of biopolymer impregnated with antibodies against saliva biomarkers, and nanoparticle electronics that measures changes in tunneling current upon analyte binding. This approach enables sensitive, specific and reversible detection of neutrally charged cortisol and D-amylase without addition of chemical reporters or redox reagents for signal transduction. Such capability overcomes the limits encountered in charge-based transistor/nanowire biosensors or electrochemical biosensing techniques. A passive RFID system will be integrated into the sensor for wireless monitoring of salivary biomarkers. The study will provide insights into the science of functional materials, nanoscale electron transport, mass transport in confined geometries and device integration. Broader impacts: The goal of the proposed education and outreach effort is to foster the development of a diverse science and engineering workforce with a deep understanding of functional materials, nanotechnology, and devices for biosensing and signal transduction. To achieve this goal, the education and outreach objectives are: to develop new lab class for teaching biosensor and medical devices that allow students to conduct experiments involving, surface chemistry, molecular detection and mass transport in microfluidics, and to recruit and retain women and minorities in science and engineering careers by engaging minority undergraduate and high school students in summer research projects and through participation in our SESEY summer camp, which offers research experience in engineering fields for high school girls and minority students, and ASE program, which provides an eight-week summer apprenticeship to high school students in our research labs. A hierarchical mentorship structure is proposed in which students at each level will have the opportunity to serve as mentors for younger students.

1512816 Cheng，这项研究将开发出一种新型的传感器设备，该设备将能够测量当一个人处于心理压力下时在唾液中发现的分子。 该研究如果成功将能够在门诊环境中测量应力标记。 由于生物传感器将配备无线连接，因此可以远程监测个人所经历的压力。实时，检测压力唾液生物标志物可以评估个体的压力水平。通常通过荧光免疫测定和酶动力学方法分析唾液生物标志物，无法原位进行。拟议的压力生物传感器以可穿戴配置建造，将大大受益于经常参与压力密集型活动的人，例如士兵，飞行员和紧急护理专业人员。通过感应功能，可以尽早检测到压力和疲劳，以避免可能造成损害。智力优点：所提出的传感器由一种生物聚合物的复合物组成，该复合物含有针对唾液生物标志物的抗体和纳米颗粒电子设备，该纳米颗粒电子设备在分析物结合后测量隧道电流的变化。这种方法可实现对中性带电的皮质醇和D-淀粉酶的敏感，特异性和可逆的检测，而无需添加化学记者或氧化还原试剂进行信号转导。这种能力克服了基于电荷的晶体管/纳米线生物传感器或电化学生物传感技术的极限。被动RFID系统将集成到传感器中，以无线监测唾液生物标志物。这项研究将提供有关功能材料科学，纳米级电子传输，庞大的几何形状和装置整合的群众传输的见解。更广泛的影响：拟议的教育和外展工作的目标是促进多样化的科学和工程劳动力的发展，并深入了解功能材料，纳米技术以及用于生物传感和信号传输的设备。为了实现这一目标，教育和外展目标是：开发新的实验室课程，以教授生物传感器和医疗设备，以使学生可以在微流体学中进行涉及，表面化学，分子检测和质量运输的实验，并招募并保留妇女和少数民族通过与少数群体的本科生和高中生参与夏季研究项目以及参加我们的Sesey夏令营，在科学和工程职业中获得研究，该夏令营为高中女生和少数族裔学生提供工程领域的研究经验，以及提供八八次 - 八级的ASE计划在我们的研究实验室的高中生夏季学徒制。提出了一个分层指导结构，每个级别的学生都有机会为年轻学生担任指导。