Collaborative Research: Injectable, Biocompatible, Programmed-Bioresorbable Nanosensor Array for In-Vivo Continuous Glucose Monitoring

合作研究：用于体内连续血糖监测的可注射、生物相容性、程序化生物可吸收纳米传感器阵列

• 批准号: 1506966
• 负责人: Hanchen Huang
• 金额: $ 9.7万
• 依托单位: Northeastern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2018-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1506966&HistoricalAwards=false
• 关键词: Collaborative; Research; Injectable; Biocompatible; Programmed

Diabetes is a metabolic disorder and major world health problem. As stated by the International Diabetes Federation, there are over 350 million diabetics worldwide causing over 3.2 million deaths annually. Consequently, glucose detection is of paramount importance in the diagnosis and treatment of diabetes. This project will illustrate how nanotechnology, biomaterials, and biosensing technologies can be meshed together for injectable, biocompatible, bioresorbable nanosensor array for continuous glucose monitoring. The work will advance in vitro and/or in vivo monitoring technology for continuous glucose monitoring in general and, if successful, will not only have an enormous impact on public health, but also provide a demonstration from basic science to applications. This project will impact education of the graduate, undergraduate and high school students by integrating advanced biosensing into their educational and laboratory training, and actively interact with the general public and industry companies through various outreach activities within and outside of the University of Connecticut and Northeastern University. Moreover, a new course of Bioanalytical Sensor will be offered to students. This multidisciplinary proposal aims to develop an injectable biocompatible and programmed-bioresorbable surface-enhanced Raman scattering sensor for long-term, subcutaneous, continuous glucose monitoring. A number of novel features crossing bio-, nano-, material- and sensing technologies will be seamlessly meshed into this novel glucose sensing system. A sandwich-like glucose sensing material, including programmed-bioresorbable glucose-responsive nanosensor array covered by biocompatible and biodegradable hydrogel with entrapped anti-inflammatory drug, will be fabricated. Then a sterilized indwelling needle will be used to deliver the as-prepared sensor subcutaneously for long-term, continuous glucose sensing. The sensing performance will be systematically investigated. The synergistic effects of (1) good biocompatibility and biodegradability of hydrogel, together with (2) good biocompatibility and stability of nanosensor array, (3) programmable bioresorbability of sensing materials after long-term use, (4) specific and reversible binding of glucose with sensing material and its unique and strong glucose-responsive optical signals distinguishable from those of skin tissue and hydrogel, (5) non-invasive monitoring through Raman scattering, (6) anti-inflammatory effect provided by anti-inflammatory drug entrapped in hydrogel, and (7) minimally invasive delivery of sensor through convenient skin piercing, will accomplish the goal of reliable long-term continuous glucose monitoring.

糖尿病是一种代谢紊乱和主要的世界健康问题。据国际糖尿病联盟称，全球有超过 3.5 亿糖尿病患者，每年造成超过 320 万人死亡。因此，血糖检测对于糖尿病的诊断和治疗至关重要。该项目将说明如何将纳米技术、生物材料和生物传感技术结合在一起，形成用于连续血糖监测的可注射、生物相容性、生物可吸收的纳米传感器阵列。这项工作将总体上推进连续血糖监测的体外和/或体内监测技术，如果成功，不仅将对公众健康产生巨大影响，而且还将提供从基础科学到应用的示范。该项目将通过将先进的生物传感融入到他们的教育和实验室培训中来影响研究生、本科生和高中生的教育，并通过康涅狄格大学和东北大学内外的各种外展活动与公众和行业公司积极互动。此外，还将为学生开设生物分析传感器新课程。这项多学科提案旨在开发一种可注射的生物相容性和程序化生物可吸收表面增强拉曼散射传感器，用于长期皮下连续血糖监测。跨越生物、纳米、材料和传感技术的许多新颖功能将无缝融入到这种新型葡萄糖传感系统中。将制造一种三明治状的葡萄糖传感材料，包括程序化生物可吸收葡萄糖响应纳米传感器阵列，其上覆盖有生物相容性和可生物降解的水凝胶，并包埋有抗炎药物。然后，将使用消毒的留置针将准备好的传感器皮下输送，以进行长期、连续的葡萄糖传感。将系统地研究传感性能。 (1)水凝胶良好的生物相容性和生物降解性，与(2)纳米传感器阵列良好的生物相容性和稳定性，(3)长期使用后传感材料的可编程生物再吸收性，(4)葡萄糖的特异性和可逆结合的协同效应具有传感材料及其独特的强葡萄糖响应光信号，可区别于皮肤组织和水凝胶的光信号，（5）通过拉曼散射进行无创监测，（6）抗炎作用水凝胶中包埋的抗炎药物，以及（7）通过方便的皮肤穿刺微创输送传感器，将实现可靠的长期连续血糖监测的目标。