US-Taiwan Planning Visit: Interrogating Nanodiamond-Cellular Interactions

美国-台湾计划访问：探讨纳米金刚石与细胞的相互作用

• 批准号: 1444100
• 负责人: Dean Ho
• 金额: $ 6.71万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-15 至 2017-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1444100&HistoricalAwards=false
• 关键词: US; Taiwan; Planning; Visit; Interrogating

The goal of this proposal is to bring together expertise from researchers in the US and Taiwan to study how nanodiamonds, promising carbon particles that have been shown to markedly improve the safety and efficiency of molecular imaging and other applications, fundamentally interact with biological systems. The ability to examine the basic biological response to novel materials remains an important need in the bioengineering, materials science, chemistry and engineering education communities, among many others. The information gleaned from these studies can improve materials design principles for a broad array of applications. This work will enable rational design and chemical modification of the unique nanodiamond surfaces to realize improved biocompatibility and insight into how cells respond following exposure to these agents. This study will result in broad impact upon improving the safety and thus widespread implementation of nanodiamonds.A major part of this project is developing novel K-12 teaching materials, specifically a nanodiamond-themed comic book. Graphic materials are a great way to facilitate student learning of complex topics, and will help inspire a new generation to explore future careers in science and engineering. Specifically, a team of graduate students and the academic coordinator of UCLA will partner with Taiwanese collaborators to create the Nanodiamond Nora and Neal storyline. The Nanodiamond Nora and Neal will serve as the main characters of the book which will highlight their story from serving as engine abrasives to journeying into cells as explorers. Nora and Neal will be mentored by young scientists during the course of their journey. Technical AbstractNanodiamonds have emerged as promising materials for fundamental biological investigations. The faceted nature of the truncated octahedral nanodiamond surfaces results in unique physical-chemical properties that mediate marked improvements in the efficacy and safety of molecular imaging agents. These properties include the ability to coordinate water, a key requirement for optimizing imaging contrast. As such, nanodiamond-based imaging has uniquely resulted in among the highest efficiencies compared to all nanoparticle compounds. Given their promise as nano-labeling materials, it is becoming increasingly important to better understand the mechanisms that underlie nanodiamond-cell interactions. This work aims to bring together a team of collaborators to provide the most comprehensive study to date of how broad classes of nanodiamonds (amine, carboxyl-functionalized, fluorescent/N-V center, etc.) interact with cells. Methodologies such as caspase assays, quantitative real-time polymerase chain reaction, viability assays, and lactate dehydrogenase assays will be implemented to provide new fundamental mechanistic insight into the biological response to particle exposure. Furthermore, CyTOF, a powerful single cell investigation platform that enables multi-pathway interaction assays will provide the most elaborate view to date of how cell signaling components crosstalk to collectively govern cell behavior. This work will play a major role in technically training future scientific and engineering leaders.

该提案的目的是将美国和台湾研究人员的专业知识汇总在一起，以研究纳米登录，有望有望明显提高分子成像和其他应用的安全性和效率的有希望的碳颗粒，并从根本上与生物系统相互作用。在生物工程，材料科学，化学和工程教育社区等等，检查对新材料的基本生物学反应的能力仍然是重要的需求。从这些研究中收集的信息可以改善广泛应用的材料设计原理。这项工作将使独特的纳米原子表面的合理设计和化学修饰能够实现改善的生物相容性，并洞悉暴露于这些药物后细胞如何反应。这项研究将对改善安全性并因此广泛实施纳米原子。该项目的主要部分是开发新型的K-12教学材料，特别是以纳米amond为主题的漫画书。图形材料是促进学生学习复杂主题的好方法，并将有助于激发新一代探索科学和工程学的未来职业。具体来说，一支由研究生组成的团队和UCLA的学术协调员将与台湾合作者合作，创建纳米原子诺拉和尼尔故事情节。 Nanodiamond Nora和Neal将充当本书的主要角色，这些人物将重点介绍他们的故事，从作为发动机磨料到作为探险者的牢房。在旅途中，诺拉（Nora）和尼尔（Nora）将受到年轻科学家的指导。技术摘要纳米蒙德人已成为基本生物学研究的有前途的材料。截短的八面体纳米座表面的刻面性质导致独特的物理化学特性，介导了分子成像剂的功效和安全性的明显改善。这些特性包括协调水的能力，这是优化成像对比度的关键要求。因此，与所有纳米颗粒化合物相比，基于纳米梁的成像在最高效率中产生了唯一的效率。鉴于他们作为纳米标记材料的承诺，更好地了解纳米木纤维相互作用的机制变得越来越重要。这项工作旨在将一组合作者团队汇集在一起​​，以提供迄今为止最全面的研究，以了解广泛类别（胺，羧基功能化，荧光/N-V中心等）如何与细胞相互作用。将实施诸如caspase分析，定量实时聚合酶链反应，生存力测定和乳酸脱氢酶测定等方法论，以提供对生物学对颗粒暴露的生物学反应的新基本机械洞察力。此外，Cytof是一个强大的单细胞研究平台，可以实现多条纹相互作用测定法，将为迄今为止的细胞信号传导组件如何串扰以统一控制细胞行为的方式提供最精致的视图。这项工作将在技术培训未来的科学和工程领导者中发挥重要作用。