SBIR Phase II: An Intelligent Rapid Prototyping System for Synthetic Biology

SBIR 第二阶段：合成生物学智能快速原型系统

• 批准号: 1430986
• 负责人: Michael Fero
• 金额: $ 75万
• 依托单位: TeselaGen Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-10-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1430986&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Intelligent; Rapid

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase II project will be to accelerate the pace of microbe development for the biomanufacture of valuable proteins, advanced enzymes for industry, or therapeutic medicines. DNA cloning is an everyday practice in the course of both industrial- and university-based research. Cloning technology has remained largely unchanged for the last 20 years. As a consequence, researchers consume a significant amount of time and money designing and constructing DNA, rather than on designing and conducting experiments. Over the past few years, standardized experimental DNA construction methods have been developed that lend themselves well to automation and rapid assembly of DNA. Process automation is progressing from luxury to necessity, as target applications demand the fabrication of large combinatorial DNA libraries in the search for better antibodies, faster enzymes, and more productive microbial strains. The proposed technology will allow rapid forward engineered biological libraries of recombinant DNA. The commercial availability of this technology will provide a low cost alternative to current methods.This SBIR Phase II project aims to develop a bioCAD/CAM (Computer Aided Design and Manufacturing) technology that enables rapid DNA assembly for synthetic biology. Wedding recent advances in DNA assembly methods, and the software algorithms used to design those DNA assemblies, the proposed research will result in a platform technology for facilitating an optimized combination of direct synthesis and DNA assembly to make large combinatorial libraries. After the construction of the DNA libraries, screening for constructs with the desired activity remains a major scale-limiting bottleneck, both in terms of cost and time. The proposed technology will allow rapid prototyping and characterization of forward engineered biological libraries of recombinant DNA, proteins, or whole cells. The goal is to commercialize an easy-to-use platform for assembling complex constructs onto vector backbones, transfecting them into host microbes, and doing a rapid assessment of product yield. Results are captured, saved, and returned to a design database after data cleansing and analysis.

这项小型企业创新研究（SBIR）第二阶段项目的更广泛的影响/商业潜力将是加快微生物开发的速度，以生物制造有价值的蛋白质，高级工业酶或治疗性药物。在基于工业和大学的研究过程中，DNA克隆是日常实践。在过去的20年中，克隆技术在很大程度上保持不变。 结果，研究人员消耗了大量的时间和金钱设计和构建DNA，而不是设计和进行实验。在过去的几年中，已经开发出标准化的实验性DNA构建方法，非常适合自动化和快速组装DNA。过程自动化正在从奢侈品到必要性发展，因为目标应用要求在寻找更好的抗体，更快的酶和更有效的微生物菌株时制造大型组合DNA库。拟议的技术将允许重组DNA的快速前进工程生物库。该技术的商业可用性将为当前方法提供低成本的替代方案。该SBIR II期项目旨在开发生物AD/CAM（计算机辅助设计和制造）技术，该技术可为合成生物学提供快速的DNA组装。 DNA组装方法的最新进展以及用于设计DNA组件的软件算法，拟议的研究将导致平台技术，以促进直接合成和DNA组件的优化组合，以制造大型组合库。 在构建DNA库后，在成本和时间方面，筛选具有所需活动的构造仍然是限制性的瓶颈。 所提出的技术将允许快速原型制作和表征重组DNA，蛋白质或全细胞的正向工程生物库。 目的是将一个易于使用的平台商业化，用于将复杂构造组装到矢量骨架上，将其转染到宿主微生物中，并对产品产量进行快速评估。 在数据清理和分析后，捕获，保存，保存并返回到设计数据库。