SBIR Phase I: A novel biological method to suppress Bacillus thuringiensis (Bt) Toxin resistance

SBIR 第一阶段：抑制苏云金芽孢杆菌 (Bt) 毒素抗性的新型生物方法

• 批准号: 1415524
• 负责人: Angelika Fath-Goodin
• 金额: $ 15万
• 依托单位: Paratechs Corp.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1415524&HistoricalAwards=false
• 关键词: SBIR; Phase; novel; biological; method

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase I project is the development of a technology to sustain the efficacy of transgenic plants expressing the Bacillus thuringiensis (Bt) toxin. Transgenic expression of Bt toxins is a major tool for the control of insect pests in agriculturally important crops. Bt transgenic technology has been deployed on over one billion acres because it improves crop yields and reduces the environmental problem of using toxic chemical pesticides. Insects, however, are becoming increasingly resistant to the Bt toxin, which is threatening its sustainability. This project proposes to develop alternative means to suppress populations of important lepidopteran pest(s) targeted by Bt. There is no greater threat to sustainability of the Bt technology than the development of resistance, with current evidence indicating that resistance is increasingly impacting producers globally. Novel strategies to suppress or eliminate insect populations resistant to Bt represent a significant commercial opportunity. This SBIR Phase I project proposes to develop a novel biological control agent for use in specifically suppressing insect populations that are developing resistance to the Bt toxin. This project will produce a biological control agent, and test it for the ability to replicate in the target insect and determine if it has the predicted pathology. Once the agent is developed and its properties evaluated in the laboratory, experiments will be performed to establish methods for efficient infection of target pests. Phase II will assess impacts on pest populations in microcosm experiments with data used to develop population models of effects in the field. Successful experimental results will develop the technology to the point of field testing and commercialization planned for Phase III.

这项小型企业创新研究（SBIR）I期项目的更广泛的影响/商业潜力是开发一种技术来维持表达苏云金芽孢杆菌（BT）毒素的转基因植物的功效。 BT毒素的转基因表达是控制农业重要农作物中虫害的主要工具。 BT转基因技术已被部署在10亿英亩的土地上，因为它提高了作物的产量并减少了使用有毒化学农药的环境问题。然而，昆虫正变得越来越抗BT毒素，这威胁到其可持续性。该项目提议开发替代手段，以抑制BT针对的重要鳞翅目害虫的种群。 BT技术对可持续性的威胁没有比阻力的发展更大的威胁，目前的证据表明，阻力在全球范围内越来越多地影响生产者。抑制或消除抵抗BT的昆虫种群的新型策略代表了重要的商业机会。 该SBIR I期项目提议开发一种新型的生物控制剂，用于专门抑制正在发展对BT毒素的抗性的昆虫种群。该项目将产生生物控制剂，并测试其在靶昆虫中复制并确定其是否具有预测病理的能力。一旦开发了代理并在实验室中评估其性能，将进行实验以建立有效感染靶病虫害的方法。第二阶段将评估对缩影实验中有害生物种群的影响，该数据用于开发现场效应人群模型的数据。成功的实验结果将开发该技术，直到计划为III期计划的现场测试和商业化。