EAGER: Small molecule regulation of plant autophagy and the biochemical characterization of whole intact autophagosomes

EAGER：植物自噬的小分子调控和完整自噬体的生化特征

• 批准号: 1355459
• 负责人: Savithramma Dinesh-Kumar
• 金额: $ 30万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-11-15 至 2015-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1355459&HistoricalAwards=false
• 关键词: EAGER; Small; molecule; regulation; plant

Autophagy, the regulated degradation and recycling of cellular material, is required for many cellular functions including the response to starvation, differentiation and development and senescence and immunity. However, the regulation of this process and the structure of the autophagosome (the organelle in which autophagy occurs) are not well understood in plants. This project will advance our understanding of autophagy in plants (specifically Arabidopsis) by addressing two major unsolved challenges 1) developing high-throughput assays to identify small chemical modulators of autophagy in plants, and 2) developing methods for the biochemical purification and study of plant autophagosomes. Broader Impacts. Training at the intersection of proteomics, biochemistry, cell biology and chemical genomics will be provided to a postdoctoral fellow and to undergraduate students.

自噬是细胞材料的调节降解和回收利用，需要许多细胞功能，包括对饥饿，分化，发育以及衰老和免疫力的反应。然而，在植物中，该过程的调节和自噬体的结构（自噬发生的细胞器）尚不清楚。该项目将通过解决两个主要未解决的挑战（特别是拟南芥）中的自噬的理解。更广泛的影响。将向博士后研究员和本科生提供蛋白质组学，生物化学，细胞生物学和化学基因组学交集的培训。