COLLABORATIVE RESEARCH: HIGH-SPEED AFM IMAGING OF DYNAMICS ON BIOPOLYMERS THROUGH NON-RASTER SCANNING

合作研究：通过非光栅扫描对生物聚合物动力学进行高速 AFM 成像

• 批准号: 1353101
• 负责人: Kam Leang
• 金额: $ 23.67万
• 依托单位: Board of Regents, NSHE, obo University of Nevada, Reno
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-15 至 2014-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1353101&HistoricalAwards=false
• 关键词: COLLABORATIVE; RESEARCH; SPEED; AFM; IMAGING

This award by the Instrument Development for Biological Research (IDBR) program in the Division of Biological Infrastructure (BIO Directorate) is co-funded by the Particulate and Multiphase Processes (PMP) program in the Division of Chemical, Bioengineering, Environmental, and Transport Systems (CBET, Engineering Directorate).Non Technical AbstractThe primary aim of this project is to create a novel high-speed atomic force microscope (AFM) imaging system with frame rates of on the order of 100 frames/second. While the creation of this instrument may have a significant impact on a broad range of application areas, the primary target is the study of biomolecular processes. The extremely fast frame rate and long range will allow for the direct visualization of dynamic process that previously could be studied at best only through indirect means. The interdisciplinary nature of the project provides excellent opportunities for both graduate and undergraduate student training as well as outreach to middle- and high-school age students through summer programs on nano- and biotechnology.Technical AbstractThe speed gains of the instrument will be achieved in two ways. The first way is to replace the standard raster-scan of AFM with a feedback algorithm that steers the tip to stay in the region of interest. This will reduce imaging time by reducing the amount of scanning to be done, with the tradeoff that the sample to be imaged must have an underlying string-like structure such as is found in biopolymers. The second way is to create dual-stage scanning systems that will allow the non-raster scanning to be performed at ultra-fast speeds. A prototype system will be built and demonstrated on a variety of standard samples before being deployed to study the dynamics of the molecular motor myosin V. The instrument and underlying techniques will be disseminated through journal publication and conference publication and through collaboration with an existing AFM company to integrate the techniques in their next generation devices.

该奖项由生物基础设施部 (BIO 理事会) 生物研究仪器开发 (IDBR) 计划颁发，并由化学、生物工程、环境和运输系统部的颗粒和多相过程 (PMP) 计划共同资助（CBET，工程理事会）。非技术摘要该项目的主要目标是创建一种帧速率约为 100 帧/秒的新型高速原子力显微镜 (AFM) 成像系统。虽然该仪器的创建可能对广泛的应用领域产生重大影响，但主要目标是生物分子过程的研究。极快的帧速率和远距离将允许动态过程的直接可视化，而以前只能通过间接手段进行研究。该项目的跨学科性质为研究生和本科生培训以及通过纳米和生物技术暑期课程向初中和高中年龄的学生提供了极好的机会。技术摘要该仪器的速度增益将在两个方面实现方式。第一种方法是用反馈算法取代 AFM 的标准光栅扫描，引导尖端停留在感兴趣的区域。这将通过减少要完成的扫描量来缩短成像时间，但代价是要成像的样品必须具有潜在的线状结构，例如在生物聚合物中发现的结构。第二种方法是创建双级扫描系统，允许以超快的速度执行非光栅扫描。原型系统将在各种标准样品上进行构建和演示，然后用于研究分子运动肌球蛋白 V 的动力学。该仪器和基础技术将通过期刊出版物和会议出版物以及与现有 AFM 公司的合作进行传播将这些技术集成到他们的下一代设备中。