Impact of Alternative Substrates on Methane, Methanol, and Chloromethane Metabolism of Methylotrophs in Forest Soils

替代基质对森林土壤中甲基营养菌的甲烷、甲醇和氯甲烷代谢的影响

基本信息

批准号：
212127456
负责人：
Professor Dr. Steffen Kolb
金额：
--
依托单位：
Lehrstuhl für Ökologische Mikrobiologie (ÖMIK)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2012
资助国家：
德国
起止时间：
2011-12-31 至 2017-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/212127456?language=en
关键词：
Impact Alternative Substrates Methane Methanol

项目摘要

Methylotrophic microbes in forest soils govern to a substantial degree the global exchange of methane, methanol, and chloromethane of terrestrial ecosystems with the atmosphere. Methane is a greenhouse gas. Methanol and chloromethane affect ozone formation. Forest soil methylotrophs utilise these compounds as energy and carbon sources. However, ambient concentrations of methane, methanol, and chloromethane are such low that it is unlikely that their utilisation allows for sufficient energy conservation of methylotrophs. Thus, forest soil methylotrophs likely utilise alternative substrates that are abundant in forest soil, such as hydrolysis products of cellulose and hemicellulose, anaerobic degradation products of organic matter, nalkanes from plant waxes, and soluble metabolites of lignin. The proposed project will resolve trophic strategies of methylotrophs that utilise one-carbon atmospheric gases, and thrive in forest soils. Soil samples of two temperate beech stands will be investigated (Steigerwald, Solling). The proposed experiments will allow for identification of active methylotrophs and their metabolic pathways at in situ-relevant substrate concentrations using state of the art stable isotope probing techniques, cultivation, metatranscriptomics and –proteomics, and substrate consumption kinetics. Furthermore, experiments will reveal insights into the impact of soil microbial communities on the yet insufficiently resolved global cycles of methanol and chloromethane.

森林土壤中的甲基营养微生物在很大程度上控制着陆地生态系统的甲烷、甲醇和氯甲烷与大气的交换。甲醇和氯甲烷影响森林土壤甲基营养微生物利用这些化合物作为能量和碳。然而，甲烷、甲醇和氯甲烷的环境浓度如此之低，以至于它们的利用不太可能充分节约能源。因此，森林土壤甲基营养菌可能会利用森林土壤中丰富的替代基质，例如纤维素和半纤维素的水解产物、有机物的厌氧降解产物、植物蜡中的正烷烃以及木质素的可溶性代谢物。解决甲基营养菌利用一碳大气气体的营养策略，并在两个温带的森林土壤样本中茁壮成长。将研究山毛榉林（Steigerwald、Solling）。拟议的实验将利用最先进的稳定同位素探测技术、培养、宏转录组学和蛋白质组学，在原位相关底物浓度下鉴定活性甲基营养菌及其代谢途径。此外，实验将揭示土壤微生物群落对尚未充分解决的甲醇和氯甲烷全球循环的影响。