Ca2+ Waves in Systemic Signaling Networks in Plants

植物系统信号网络中的 Ca2 波

基本信息

批准号：
1329723
负责人：
Simon Gilroy
金额：
$ 63.48万
依托单位：
University of Wisconsin-Madison
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2017-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1329723&HistoricalAwards=false
关键词：
Ca2+Waves Systemic Signaling Networks

项目摘要

Intellectual MeritThe immobile nature of the plant lifestyle requires an ability to continuously monitor local conditions and trigger responses to help cope as these conditions change. Stimuli are often highly localized, yet responses must occur throughout the plant body. Thus, the signaling networks within plants must broadcast information not only within the cells directly receiving the stimulus but also over much longer distances. This project seeks to define how the calcium ion plays a role in this long-distance signaling system. Preliminary work has revealed that in response to local stress, such as wounding of the leaf or increasing levels of salt around the root, waves of Ca2+ propagate from the site of stress perception to distant regions of the plant within seconds. This preliminary analysis has also highlighted a potential role for reactive oxygen species (ROS) and an ion channel named TPC1 in this rapidly propagating Ca2+ wave system. The major research goals to be undertaken in this project are therefore to use plants engineered to produce protein-based Ca2+ reporters to monitor and characterize the Ca2+ changes both at the site of stress perception and in the tissues transmitting this signal. Molecular changes dependent on the Ca2+ wave will then be monitored to define the relationship between the Ca2+ wave and other well-defined plant stress response pathways such as those triggered by ROS and the defense hormones jasmonic- and salicylic-acid. The third goal will be to engineer the TPC1 gene to alter the regulation of this ion channel such that it should no longer respond to ROS or Ca2+. The ability of these mutated versions of the channel to support the movement of the Ca2+ wave will then be assessed. Through pursuing these three goals, this research program will help define how this newly discovered Ca2+ wave signaling system plays a role in helping plants adapt to a constantly changing environment.Broader ImpactsThis project will integrate research and education. Research opportunities for undergraduates will be offered through programs such as the Undergraduate Research Scholars Program and recruitment from members of the Posse Foundation that promote the representation of underrepresented groups in science. Opportunities for high school researchers will also be made available. Along with their research training these scholars will be encouraged to present their work at local, national and international meetings. In addition, efforts at outreach to the general public will be made at the local level through events such as the Wisconsin Institutes of Discovery Science Festival, Wisconsin Science Expeditions and Saturday Science at The Wisconsin Institutes of Discovery. A plant biology movie resource, disseminated through outlets such as YouTube, will also be developed. This resource will be directed towards engaging the general public through movies and explanations of plant responses, whilst also providing them with access to the science behind the images.

知识分子值得植物生活方式的不动性质，需要能够连续监测当地条件并触发反应以帮助应对这些条件随着这些条件的变化而进行。刺激通常是高度局部的，但必须在整个植物体内发生反应。因此，植物中的信号网络不仅必须在直接接收刺激的细胞内广播信息，而且还必须在更长的距离内广播。该项目旨在定义钙离子如何在该长距离信号系统中起作用。初步工作表明，响应局部压力，例如叶片的损伤或根周围的盐水平增加，Ca2+的波从压力感知部位传播到植物的远处，几秒钟内。这种初步分析还强调了在这个迅速传播的Ca2+波系统中，活性氧（ROS）和称为TPC1的离子通道的潜在作用。因此，该项目要实现的主要研究目标是使用工程设计的植物来生产基于蛋白质的CA2+报告者，以监测和表征压力感知部位和传输该信号的组织中的Ca2+变化。然后，将监测取决于Ca2+波的分子变化，以定义Ca2+波与其他定义明确的植物应力反应途径之间的关系，例如由ROS触发的途径以及防御激素茉莉花和水杨酸酸。第三个目标是设计TPC1基因以改变该离子通道的调节，以使其不再应对ROS或Ca2+。然后将评估这些通道这些突变版本支持Ca2+波运动的能力。通过实现这三个目标，该研究计划将有助于定义新发现的CA2+波信号系统如何在帮助植物适应不断变化的环境中发挥作用。BRODERIMPACTSSSUSSPONTIONS这些项目将整合研究和教育。本科生的研究机会将通过诸如本科生研究学者计划和POSSE基金会成员的招聘等计划提供，这些计划促进了科学中代表性不足的群体的代表。高中研究人员的机会也将提供。随着他们的研究培训，这些学者将被鼓励在当地，国家和国际会议上介绍他们的工作。此外，将通过威斯康星州科学节，威斯康星州科学探险和威斯康星州发现研究所的周六科学等活动在地方一级向公众推广的努力。通过YouTube等渠道传播的植物生物学电影资源也将开发。该资源将用于通过电影和植物反应的解释来吸引公众，同时还为他们提供了图像背后的科学访问权限。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Tip Growth

DOI：
10.1002/9780470015902.a0023746
发表时间：
2017-01-01
期刊：
eLS
影响因子：
0
作者：
Gilroy, S.;Swanson, S.J
通讯作者：
Swanson, S.J

Sense and sensibility: the use of fluorescent protein-based genetically encoded biosensors in plants

DOI：
10.1016/j.pbi.2018.07.004
发表时间：
2018-12-01
期刊：
CURRENT OPINION IN PLANT BIOLOGY
影响因子：
9.5
作者：
Hilleary, Richard;Choi, Won-Gyu;Gilroy, Simon
通讯作者：
Gilroy, Simon

Control of basal jasmonate signalling and defence through modulation of intracellular cation flux capacity

DOI：
10.1111/nph.14754
发表时间：
2017-12-01
期刊：
NEW PHYTOLOGIST
影响因子：
9.4
作者：
Lenglet, Aurore;Jaslan, Dawid;Farmer, Edward E.
通讯作者：
Farmer, Edward E.

Using GCaMP3 to Study Ca2+ Signaling in Nicotiana Species

DOI：
10.1093/pcp/pcx053
发表时间：
2017-07-01
期刊：
PLANT AND CELL PHYSIOLOGY
影响因子：
4.9
作者：
DeFalco, Thomas A.;Toyota, Masatsugu;Yoshioka, Keiko
通讯作者：
Yoshioka, Keiko

Plants eavesdrop on cues produced by snails and induce costly defenses that affect insect herbivores

植物窃听蜗牛发出的信号并引发影响昆虫食草动物的昂贵防御

DOI：
10.1007/s00442-018-4070-1
发表时间：
2018
期刊：
Oecologia
影响因子：
2.7
作者：
Orrock, John L.;Connolly, Brian M.;Choi, Won-Gyu;Guiden, Peter W.;Swanson, Sarah J.;Gilroy, Simon
通讯作者：
Gilroy, Simon