New Frameworks of SSNMR for Biomolecules and Nano-assembled Systems for Sensitivity, Reaction, and Structure

用于生物分子和纳米组装系统的灵敏度、反应和结构的 SSNMR 新框架

• 批准号: 1310363
• 负责人: Yoshitaka Ishii
• 金额: $ 46万
• 依托单位: University of Illinois at Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-15 至 2017-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1310363&HistoricalAwards=false
• 关键词: New; Frameworks; SSNMR; Biomolecules; Nano

Nuclear magnetic resonance (NMR) spectroscopy is an indispensable tool for determining the structure of molecules. In this project, supported by the Chemical Measurement and Imaging Program of the Division of Chemistry, Prof. Yoshitaka Ishii of the University of Illinois at Chicago and his research group will establish a new framework of next-generation NMR spectroscopy for biomoleculces and materials in solids, which is generally called solid-state NMR (SSNMR). The proposed work would aim to increase the speeds of the data collection 50-6000 fold over a traditional method by integrating advanced methods such as 1H detection, novel isotope labeling, and paramagnetic doping into a non-traditional SSNMR approach using an ultra high magnetic field and very fast magic angle spinning. The second component of the research involves SSNMR analysis of peptides and proteins during solid-phase peptide synthesis, which reveals the structural changes of the protein during the reactions. The third component is to examine graphene/graphite based nano materials, in particular, chemical reactions of graphene oxide and graphite oxide and the interactions of H2O with porous grapheme-based systems. Ishii's research will advance NMR technology applications to a broader range of insoluble proteins and non-crystalline materials, which cannot be analyzed by other tools such as x-ray crystallography. Advances in the Ishii group research will likely have impacts in the many venues of NMR based technology, including nanomaterials analysis and peptide science. Professor Ishii will integrate his research into his educational activities by establishing a new graduate course on solid-state NMR with hands-on experiments. Professor Ishii will also incorporate a demo of a simple magnetic resonance imaging (MRI) experiment into molecular diffusion experiments with NMR field gradient in undergraduate labs in order to discuss the connection of NMR with the MRI technology used for medical diagnostics. Finally, Professor Ishii will recruit undergraduate researchers through Summer Research Opportunities for Undergraduates Program (SROP) at the University of Illinois at Chicago, which emphasizes the diversity in research-based undergraduate education.

核磁共振（NMR）光谱是确定分子结构的必不可少的工具。 在这个项目中，伊利诺伊州伊利诺伊大学芝加哥大学及其研究小组的化学测量和成像计划的支持，将建立一个新的NMR NMR NMR光谱框架的新框架，用于固体中的生物分泌和材料，通常称为固态NMR（通常称为NMR（SSNMR））。 拟议的工作将旨在通过整合1H检测，新型同位素标记和顺磁性掺杂，使用超高磁场和非常快速的魔术角旋转来提高数据收集的速度50-6000倍的传统方法，例如1H检测，新颖的同位素标记和顺磁性掺杂。 该研究的第二部分涉及固相肽合成过程中肽和蛋白质的SSNMR分析，这揭示了反应过程中蛋白质的结构变化。 第三个成分是检查基于石墨烯/石墨的纳米材料，特别是氧化石墨烯和氧化石墨的化学反应，以及H2O与多孔基于墨石的系统的相互作用。 ISHII的研究将把NMR技术应用推向更广泛的不溶性蛋白质和非晶体材料，这些材料无法通过其他工具（例如X射线晶体学）进行分析。 ISHII小组研究的进步可能会在基于NMR的技术的许多场所产生影响，包括纳米材料分析和肽科学。 Ishii教授将通过通过动手实验建立新的有关固态NMR的研究生课程来将他的研究纳入他的教育活动。 ISHII教授还将在本科实验室中与NMR场梯度进行分子扩散实验中的简单磁共振成像（MRI）实验的演示，以讨论NMR与用于医学诊断的MRI技术的联系。 最后，Ishii教授将通过伊利诺伊大学芝加哥大学的夏季研究机会（SROP）招募本科研究人员，该计划强调了基于研究的本科教育的多样性。