Creation of Tools for the Study of Oxidative Damage to Nucleic Acids

创建用于研究核酸氧化损伤的工具

• 批准号: 1309135
• 负责人: Amanda Bryant-Friedrich
• 金额: $ 30万
• 依托单位: University of Toledo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2016-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1309135&HistoricalAwards=false
• 关键词: Creation; Tools; Study; Oxidative; Damage

With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Dr. Amanda C. Bryant-Friedrich from the University of Toledo to develop chemical models systems for the study of nucleic acid damage resulting from oxidative processes. Synthetic organic chemistry along with analytical and biophysical techniques will be utilized to synthesize and evaluate both RNA and DNA derivatives that serve as precursors of reactive intermediates formed through nucleic acid damage processes. Damage to nucleic acids, particularly if not easily reparable, has broad implications in biomedical science and chemical biology, being a hallmark of oxidative stress/mutagenesis. The aim of this work is therefore to develop chemically stable substrates that can be utilized to determine damage pathways under biologically relevant conditions. Deoxyribonucleic acids (DNA) and their close cousins ribonucleic acids (RNA) are key biological substrates vital for the establishment of genetic characteristics and for the synthesis of proteins needed to carry out cellular processes. Damage to these important molecules leads to the compromise of the cell and has detrimental consequences in human health. The development of tools for the study of processes that damage DNA and RNA will allow for the experimental study of these damage processes with an eye toward better understanding the consequences of oxidative stress and the etiology of disease. This pursuit will allow undergraduates, graduate students and postdoctoral fellows from a broad range of backgrounds to acquire specialized training in biological chemistry. Women and minorities will be active participants in the research and training made possible through this award. The recruitment and retention of minority scientists has been and will continue to be a significant area of focus of this laboratory.

通过该奖项，化学过程的化学过程计划是为托莱多大学的Amanda C. Bryant-Friedrich博士提供资金，以开发化学模型系统，以研究氧化过程引起的核酸损害。 合成有机化学以及分析和生物物理技术将用于合成和评估RNA和DNA衍生物，这些RNA和DNA衍生物是通过核酸损伤过程形成的反应性中间体的前体。 对核酸的损害，尤其是如果不容易偿还的话，对生物医学科学和化学生物学具有广泛的影响，是氧化应激/诱变的标志。 因此，这项工作的目的是开发化学稳定的底物，这些底物可用于确定在生物学相关条件下的损伤途径。 脱氧核糖核酸（DNA）及其近乎表亲的核糖核酸（RNA）是对建立遗传特征和合成进行细胞过程所需的蛋白质至关重要的关键生物学底物。 对这些重要分子的损害导致细胞的妥协，并对人类健康产生不利的后果。开发研究损害DNA和RNA的过程的工具，将允许对这些损伤过程进行实验研究，以更好地了解氧化应激和疾病病因的后果。 这项追求将允许本科生，研究生和博士后研究员从广泛的背景中获得生物化学专业培训。妇女和少数民族将成为研究和培训的积极参与者，这是通过该奖项实现的。 少数民族科学家的招聘和保留一直是并将继续成为该实验室的重点重点领域。