CAREER: Glycosylation as a primary contributor to cell fate-specific membrane capacitance

职业生涯：糖基化是细胞命运特异性膜电容的主要贡献者

基本信息

批准号：
1254060
负责人：
Lisa Flanagan
金额：
$ 83万
依托单位：
University of California-Irvine
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2013
资助国家：
美国
起止时间：
2013-06-15 至 2018-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1254060&HistoricalAwards=false
关键词：
CAREER Glycosylation primary contributor cell

项目摘要

Neural stem cells in the central nervous system form specialized cells (neurons, astrocytes, and oligodendrocytes) to build the brain and spinal cord. Unfortunately, studying the cells that make each of these specialized cell types has been complicated because they are difficult to distinguish from one another. Through interdisciplinary studies combining biology with biophysics, Dr. Flanagan's group has found novel differences defining these cells, which can be used to obtain pure populations of each cell type for further study. The project will use cell biological, biophysical, and molecular experiments to identify key characteristics of cells forming neurons, astrocytes, and oligodendrocytes. These studies will uncover important differences in these cells and help scientists understand how the formation of the brain and spinal cord is controlled. A firm grasp on the mechanisms driving the development of the brain and spinal cord will lead to a better understanding of central nervous system disorders. Truly interdisciplinary studies such as these have the potential to unlock many biological mysteries. While interdisciplinary research has been recognized as important, it has been harder to accomplish. This project is firmly committed to interdisciplinary science, and the proposed activities will contribute to preparing the scientists of tomorrow that will need to be trained to think beyond disciplinary boundaries. This project will expose students at multiple levels (K-12, undergraduate, and graduate) to the importance of interdisciplinary research since studies coupling biology with fields such as engineering and biophysics will be critical for future progress in the biological sciences.

中枢神经系统中的神经干细胞形成专门的细胞（神经元，星形胶质细胞和少突胶质细胞），以建立大脑和脊髓。不幸的是，研究使每种专业细胞类型的细胞都变得复杂，因为它们很难彼此区分。通过将生物学与生物物理学相结合的跨学科研究，弗拉纳根博士的小组发现了定义这些细胞的新差异，这些差异可用于获取每种细胞类型的纯种群以进行进一步研究。该项目将使用细胞生物学，生物物理和分子实验来确定形成神经元，星形胶质细胞和少突胶质细胞的细胞的关键特征。这些研究将发现这些细胞的重要差异，并帮助科学家了解如何控制大脑和脊髓的形成。坚定地掌握了推动大脑和脊髓发展的机制，将使人们更好地了解中枢神经系统疾病。诸如此类的真正跨学科研究有可能释放许多生物学之谜。尽管跨学科研究被认为是重要的，但很难完成。该项目坚定地致力于跨学科科学，拟议的活动将有助于为明天的科学家做好准备，这些科学家将需要接受培训以超越学科界限。该项目将使学生在多个级别（K-12，本科生和研究生）中暴露于跨学科研究的重要性，因为研究与工程和生物物理学等领域的研究对生物科学的未来进步至关重要。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

It's Electric: When Technology Gives a Boost to Stem Cell Science

DOI：
10.1007/s40778-018-0124-x
发表时间：
2018-06-01
期刊：
CURRENT STEM CELL REPORTS
影响因子：
1.4
作者：
Lee, Abraham P.;Aghaamoo, Mohammad;Flanagan, Lisa A.
通讯作者：
Flanagan, Lisa A.

Increasing label-free stem cell sorting capacity to reach transplantation-scale throughput

DOI：
10.1063/1.4902371
发表时间：
2014-11-01
期刊：
BIOMICROFLUIDICS
影响因子：
3.2
作者：
Simon, Melinda G.;Li, Ying;Flanagan, Lisa A.
通讯作者：
Flanagan, Lisa A.

Stretch-activated ion channel Piezo1 directs lineage choice in human neural stem cells

DOI：
10.1073/pnas.1409802111
发表时间：
2014-11-11
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Pathak, Medha M.;Nourse, Jamison L.;Tombola, Francesco
通讯作者：
Tombola, Francesco