Identifying and Understanding Connections between Photosynthesis and Amino Acid Metabolism

识别和理解光合作用与氨基酸代谢之间的联系

• 批准号: 1244008
• 负责人: Yan Lu
• 金额: $ 165.33万
• 依托单位: Western Michigan University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-01 至 2018-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1244008&HistoricalAwards=false
• 关键词: Identifying; Understanding; Connections; between; Photosynthesis

Intellectual MeritPhotosynthesis provides food, fuel, and fiber to humans and other animals. The growing human population and the increasing worldwide demand for high protein diets and biofuels generate needs for higher plant productivity and improved nutritional quality in crop plants. To improve crop yield and nutritional value, it is important to have a better understanding of how plants utilize carbon from the air and nitrogen from the soil to produce biomass. In green tissues of plants, photosynthesis and the biosynthesis of many amino acids both occur in chloroplasts. These processes are linked to each other, as photosynthesis provides both the carbon and energy for amino acid biosynthesis, while amino acids are the building blocks for the protein components of photosynthesis. Despite an awareness of this relationship, large gaps exist in our understanding of how these important biological processes interact. The long-term goal of this project is to understand the connections between photosynthesis and amino acid metabolism. Computational integration of Arabidopsis thaliana phenotype data (photosynthetic parameters, amino acid and fatty acid profiles, seed carbon and nitrogen content) of mutants with defects in chloroplast-targeted nuclear-encoded gene products will be used to generate a list of candidate genes that can guide us to those connections. The candidate genes will be experimentally tested for changes in photosynthesis and amino acid metabolism, and those mutants with robust and interesting phenotypes will then undergo more focused studies. By increasing our understanding of the complex interactions between photosynthesis and amino acid metabolism, we will be in a position to effectuate advantageous changes in both. Broader ImpactsThis project will increase our understanding of the interactions between photosynthesis, carbon metabolism, and nitrogen metabolism, and these results should inform plant breeding and biotechnological approaches to crop improvement. The data and outcomes of the project will be made publicly available via publications and a project website. In addition to providing year-round training opportunities to graduate and undergraduate students, the project will integrate research and education as part of established summer Research Experiences for Undergraduate Programs at Western Michigan University (WMU) and Michigan State University (MSU). The collaboration between WMU and MSU with respect to the research, education, and outreach activities will also provide cross-disciplinary, cross-institutional training and experience to trainees in both laboratories.

智力优点光合作用为人类和其他动物提供食物、燃料和纤维。 不断增长的人口以及全球对高蛋白饮食和生物燃料日益增长的需求产生了对更高植物生产力和改善作物营养质量的需求。 为了提高作物产量和营养价值，更好地了解植物如何利用空气中的碳和土壤中的氮来产生生物量非常重要。 在植物的绿色组织中，光合作用和许多氨基酸的生物合成都发生在叶绿体中。 这些过程相互关联，因为光合作用为氨基酸生物合成提供碳和能量，而氨基酸是光合作用蛋白质成分的组成部分。 尽管认识到这种关系，但我们对这些重要的生物过程如何相互作用的理解仍存在很大差距。 该项目的长期目标是了解光合作用和氨基酸代谢之间的联系。 对叶绿体靶向核编码基因产物中存在缺陷的突变体的拟南芥表型数据（光合参数、氨基酸和脂肪酸谱、种子碳和氮含量）进行计算整合，将用于生成可以指导的候选基因列表我们与这些联系。 候选基因将通过实验测试光合作用和氨基酸代谢的变化，而那些具有强大和有趣表型的突变体将进行更有针对性的研究。 通过增加我们对光合作用和氨基酸代谢之间复杂相互作用的理解，我们将能够实现两者的有利变化。更广泛的影响该项目将增进我们对光合作用、碳代谢和氮代谢之间相互作用的理解，这些结果将为植物育种和作物改良的生物技术方法提供信息。 该项目的数据和结果将通过出版物和项目网站公开发布。 除了为研究生和本科生提供全年培训机会外，该项目还将整合研究和教育，作为西密歇根大学 (WMU) 和密歇根州立大学 (MSU) 本科生项目夏季研究经验的一部分。 西密歇根大学和密歇根州立大学在研究、教育和推广活动方面的合作还将为两个实验室的学员提供跨学科、跨机构的培训和经验。