MRI: Acquisition of an Illumina HiSeq 2000

MRI：购买 Illumina HiSeq 2000

• 批准号: 1229361
• 负责人: William Thomas
• 金额: $ 81.45万
• 依托单位: University of New Hampshire
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1229361&HistoricalAwards=false
• 关键词: MRI; Acquisition; Illumina; HiSeq; 2000

A high throughput (HTP) sequencing system will be acquired by the University of New Hampshire (UNH) to enable Northern New England research groups using genome-enabled technologies to explore basic questions in ecology, evolution, and biodiversity. This research will catalyze the generation of new interdisciplinary knowledge of fundamental biological processes and advance the role of genomics and bioinformatics across traditional biological disciplines. The instrument, an Illumina HiSeq 2500, is both fast and flexible, suited to a large array of applications. It will support the research and training programs of 16 existing laboratories at UNH, four new UNH Assistant Professors, and 8 research groups at regional undergraduate institutions using genomics to address the basic biological question: ?How do organisms respond to changes in their environment?? Users will benefit from this local, well-managed, shared instrument through (1) dramatic reductions in wait times for sample analyses, (2) intensive sample management to contain costs and maximize data output, and (3) comprehensive onsite technical and bioinformatics support. The Illumina HiSeq 2500 will be housed in the UNH Hubbard Center for Genome Studies (HCGS), an established DNA core facility with a service-driven technical, management, and bioinformatics team. The HCGS also serves as a regional bioinformatics core facility, meeting the bioinformatics and connectivity needs of scientists throughout northern New England. Management, storage, and security of the data generated by the HTP sequencing system will be provided by a dedicated IT team and infrastructure in the UNH Research Computing and Instrumentation Core Facility. To interpret and utilize the data effectively, two full-time Ph.D.-level bioinformatics staff will be dedicated to HTP sequence analysis. In addition, the HCGS works extensively with computer science faculty and students to promote the cross-disciplinary development of bioinformatics expertise. This team approach and infrastructure will ensure the efficient operation of the sequencing platform and will allow its use by the broadest number of research programs.UNH is a national leader in promoting research experiences for undergraduates; the anticipated major users of this HTP sequencing system exemplify this commitment in their own research programs. The new sequencing system will support such research experiences for undergraduate students, and also provide research opportunities for graduate students and postdoctoral fellows. To complement and support the research activities, a series of workshops will be held to provide training for students (and faculty) in HTP sequencing applications and the associated bioinformatics tools. In addition, data generated by this system will be integrated into courses in the biology curricula of participating institutions, supporting the adoption of these technologies in diverse fields. The research programs that will use this instrument already have a strong record of participation by diverse students and faculty, attracting students through programs such as the McNair Scholars, UNH Project SMART, and UNH REAP, and building faculty partnerships through collaborative research projects with historically black colleges and universities. This participation is expected to continue, and grow, as the new HTP sequencing system facilitates exploration of new areas of genomics and the application of informatics to answering a wider range of research questions.

新罕布什尔大学（UNH）将获得高通量（HTP）测序系统，以使新英格兰研究小组使用支持基因组的技术来探索生态，进化和生物多样性的基本问题。 这项研究将促进基本生物过程的新跨学科知识的产生，并在传统的生物学学科中推进基因组学和生物信息学的作用。 该乐器是Illumina Hiseq 2500，既快速又灵活，适合大量应用。 它将使用基因组学以解决基本生物学问题的区域本科机构的4个新的UNH助理教授和8个研究小组的研究和培训计划，以解决基本生物学问题：？ 用户将通过（1）等待样本分析的等待时间，（2）密集的样本管理以包含成本并最大化数据输出，以及（3）全面的现场技术和生物信息学支持。 Illumina HISEQ 2500将安置在UNH Hubbard基因组研究中心（HCGS），这是一个既定的DNA核心设施，由服务驱动的技术，管理和生物信息学团队。 HCG还可以充当区域生物信息学核心设施，满足新英格兰北部科学家的生物信息和连通性需求。 HTP测序系统生成的数据的管理，存储和安全将由UNH研究计算和仪器核心设施中的专用IT团队和基础架构提供。 为了有效地解释和利用数据，将对HTP序列分析进行两个全日制博士学位。此外，HCGS与计算机科学教师和学生广泛合作，以促进生物信息学专业知识的跨学科发展。这种团队的方法和基础设施将确保测序平台的有效运行，并允许其使用最多的研究计划。该HTP测序系统的预期主要用户在自己的研究计划中体现了这一承诺。 新的测序系统将为本科生提供此类研究经验，并为研究生和博士后研究员提供研究机会。为了补充和支持研究活动，将举办一系列研讨会，以在HTP测序应用程序和相关的生物信息学工具中为学生（和教师）提供培训。 此外，该系统生成的数据将集成到参与机构的生物学课程中，并支持在不同领域中采用这些技术。使用该工具的研究计划已经具有多样化的学生和教职员工的参与记录，通过麦克奈尔学者，UNH Project Smart和UNH收获等课程吸引学生，并通过与历史悠久的黑人学院和大学的合作研究项目建立教师合作伙伴关系。随着新的HTP测序系统有助于探索新的基因组学领域以及信息学在回答更广泛的研究问题时，预计该参与将继续并发展。