Computational and Experimental Studies of Plastid Functional Networks

质体功能网络的计算和实验研究

• 批准号: 1119778
• 负责人: Shin-Han Shiu
• 金额: $ 122.22万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1119778&HistoricalAwards=false
• 关键词: Computational; Experimental; Studies; Plastid; Functional; Computational; Experimental; Studies; Plastid; Functional

Chloroplasts are the site of photosynthesis and the defining organelle of green plants. They have the essential role of reducing atmospheric carbon dioxide, which is ultimately the source of food and feed stocks. They are also involved in the synthesis of many nutrients and specialized chemicals useful to humans, as well as playing a role in sensing changes in the environment. Most of the functions of chloroplasts (and other plastids) are carried out by proteins encoded in the nucleus, the products of the Plastid-targeted Nuclear Genes (PNGs). This project, through an integration of computational and experimental approaches, will provide a mechanistic understanding of how PNGs are regulated transcriptionally in the model plant Arabidopsis, identify what the putative roles of the PNGs are, and define how various chloroplast functions are connected. Functions of PNGs implicated in amino acid metabolic networks will be experimentally verified. These studies will lead to a better understanding of plastid biology and will also broadly inform systems approaches to biology.Broader Impacts: In addition to enhancing our understanding of regulatory and metabolic networks in plastid function, this project will continue to make use of and disseminate the findings through a publicly available phenotype database (www.plastid.msu.edu ) that is of value to the scientific community in many contexts, including research and development for genetically-engineered crop plants. In addition to providing interdisciplinary training to postdoctoral fellows and graduate students, the project will integrate research and education as part of a successful summer internship program (www.plantgenomics.msu.edu). Junior and senior undergraduate students will work in participating laboratories each summer performing research related to the project. This program will have a strong emphasis on the recruitment and training of underrepresented minorities and women.

叶绿体是光合作用和定义绿色植物细胞器的位置。它们具有减少大气二氧化碳的重要作用，这最终是食品和饲料库存的来源。他们还参与了许多营养物质和对人类有用的专业化学物质的合成，并在感知环境变化中发挥了作用。叶绿体（和其他质体）的大多数功能都是由编码在细胞核中的蛋白质（质子靶向核基因（PNG）的产物）进行的。通过计算和实验方法的整合，该项目将提供对模型植物拟南芥中PNG的转录方式的机械理解，确定PNG的推定作用是什么，并定义各种叶绿体功能如何连接。将通过实验验证与氨基酸代谢网络有关的PNG的功能。 These studies will lead to a better understanding of plastid biology and will also broadly inform systems approaches to biology.Broader Impacts: In addition to enhancing our understanding of regulatory and metabolic networks in plastid function, this project will continue to make use of and disseminate the findings through a publicly available phenotype database (www.plastid.msu.edu ) that is of value to the scientific community in many contexts, including research and development for基因工程作物植物。除了为博士后研究员和研究生提供跨学科培训外，该项目还将整合研究和教育，作为成功的暑期实习计划（www.plantgenomics.msu.edu）的一部分。初中和高级本科生将每年夏天从事与该项目有关的研究。该计划将非常重视招募和培训代表性不足的少数民族和妇女。