Continuous Ethanol Fermentation and Recovery Using an Improved Zeolite Membrane Bioreactor

使用改进的沸石膜生物反应器连续乙醇发酵和回收

• 批准号: 1067684
• 负责人: David Nielsen
• 金额: $ 36.24万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1067684&HistoricalAwards=false
• 关键词: Continuous; Ethanol; Fermentation; Recovery; Using; Continuous; Ethanol; Fermentation; Recovery; Using

PI: Nielsen, David Institution: Arizona State University Proposal Number: 1067684Title: Continuous Ethanol Fermentation and Recovery using an ImprovedZeolite Membrane BioreactorThis research aims to develop a lab-scale bioprocess which integrates fermentation with zeolite membrane pervaporation for the high-rate, high-purity, and continuous production of biofuel ethanol. To support this objective, the research is composed of three principal aims: 1) the synthesis and development of improved, aluminum-free hydrophobic zeolite membranes with both elevated ethanol permselectivity and flux, 2) characterization of non-ideal physical/biological phenomena occurring at the membrane surface, and 3) the design, development, and characterization of novel bioprocesses incorporating zeolite membrane pervaporation as a means of economical, in situ product recovery.Intellectual Merit: The first principal aim focuses on the synthesis of high quality, aluminum-free silicalite membranes on chemically inert porous zirconia supports. These new membranes, which will be fabricated in both disk and tubular geometries, are expected to demonstrate much higher fluxes and ethanol/water selectivity than existing polymer and zeolite membranes. The second important aim is to provide fundamental insights into the mechanistic nature of non-ideal, physical and/or biological interactions occurring upon zeolite surfaces, as well as their resultant effect on membrane properties and impact on separation performance. These studies will help to develop a mechanistic understanding of biofouling (by both proteins and whole cells) as a function of relevant process conditions and biocatalyst genotype. The final aim is to design, develop, and characterize a scalable and integrated bioprocess for the continuous production and recovery of ethanol from yeast fermentations using zeolite membrane pervaporation. To support sustained and high-level performance, operating policies and fouling reduction strategies, to include in situ regeneration by membrane backflushing, will be developed and optimized.Broader Impact: This study will enable developments in separation science and bioprocess design, supporting the development of sustainable biofuels. The concept of aluminum-free, silicalite membrane preparation can ultimately be translated to the synthesis of other zeolite membranes to enable greater material compatibility and higher performance for other separation applications. Newly synthesized tubular zeolite membranes and their synthesis protocols could find other applications for separation of gas and liquid mixtures of industrial importance, such as refinery gas of hydrocarbon/hydrogen and other alcohol/water mixtures. The development of a bioprocess enabling the selective separation of inhibitory products by zeolite membrane pervaporation could have broader applications for the production of additional biochemicals and emerging, next generation biofuels. The characterization of physical/biological interactions at zeolite surfaces could be valuable for assessing the future prospects of zeolite materials in fields ranging from bioenergy production to biomedical engineering. The recruited personnel and dual-use equipment resources made possible through this study will enable the development of biologically-based laboratory modules to enrich the educational experiences of undergraduates.

PI: Nielsen, David Institution: Arizona State University Proposal Number: 1067684Title: Continuous Ethanol Fermentation and Recovery using an ImprovedZeolite Membrane BioreactorThis research aims to develop a lab-scale bioprocess which integrates fermentation with zeolite membrane pervaporation for the high-rate, high-purity, and continuous production of biofuel ethanol.为了支持这一目标，该研究由三个主要目的组成：1）改进的，无铝无铝的疏水沸石膜膜的综合和发展，均具有升高的乙醇允许性和通量，2）非思想物理/生物学现象的表征，以及在膜表面上出现的备忘录，以及3）的设计，3）的特征，范围内的设计，Z）的特征，Z.作为经济，原位产品恢复的一种手段。智能优点：第一个主要目的侧重于合成高质量，无铝的无铝硅酸盐膜上化学上惰性多孔氧化锆支持的。这些新的膜将在磁盘和管状几何形状中构成，预计将比现有的聚合物和沸石膜显示出更高的通量和乙醇/水选择性。第二个重要目的是提供对沸石表面上非理想，物理和/或生物学相互作用的机械性质的基本见解，以及它们对膜性能的影响以及对分离性能的影响。这些研究将有助于发展对生物污染（蛋白质和全细胞）的机械理解，这是相关过程条件和生物催化剂基因型的函数。最终的目的是设计，开发和表征可扩展和集成的生物处理，以使用沸石膜过度蒸发从酵母发酵中连续生产和恢复乙醇。为了支持持续和高级的绩效，将开发和优化膜后冲洗的策略和减少减少策略，包括膜后冲洗的原位再生。BROADER的影响：这项研究将使分离科学和生物普罗森特设计的发展能够发展，从而支持可持续生物纤维的发展。无铝，硅石膜制剂的概念最终可以转化为其他沸石膜的合成，以使其他分离应用具有更大的材料兼容性和更高的性能。新合成的管状沸石膜及其合成方案可以找到其他用于分离工业重要性的气体和液体混合物的应用，例如碳氢化合物/氢和其他酒精/水混合物的炼油厂气体。生物过程的开发可以通过沸石膜渗透来选择性分离抑制性产物，可以在生产其他生物化学物质和新兴的下一代生物燃料中更广泛地应用。沸石表面的物理/生物相互作用的表征对于评估从生物能源生产到生物医学工程的田野中的未来前景可能是有价值的。通过这项研究获得的招募人员和双重用途的设备资源将使基于生物学的实验室模块的发展能够丰富本科生的教育经验。