Collaborative Research: Mechanistic Studies on Fe-type Nitrile Hydration Catalysts

合作研究：铁型腈水合催化剂的机理研究

• 批准号: 1058357
• 负责人: Richard Holz
• 金额: $ 19.4万
• 依托单位: Loyola University of Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-15 至 2014-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1058357&HistoricalAwards=false
• 关键词: Collaborative; Research; Mechanistic; Studies; Fe

This collaborative research award in the Chemistry of Life Processes (CLP) program supports work by Prof. Richard C. Holz of the Department of Chemistry and Biochemistry at Loyola University Chicago to carry out fundamental studies on the catalytic mechanism of iron- and cobalt-type nitrile hydratases (NHase, EC 4.2.1.84), in collaboration with Prof. Brian Bennett at the Medical College of Wisconsin (CHE-1058396). NHase's catalyze the hydration of nitriles to their corresponding commercially valuable amides in a chemo-, regio-, and/or enantio-selective manner at ambient pressures, temperatures, and physiological pH. For these reasons, NHases have attracted substantial interest as biocatalysts in preparative organic chemistry and NHase containing bacteria have found some industrial applications in the large scale production of acrylamide and nicotinamide.Since little is understood about how NHase enzymes function, a better understanding of the structure and reaction mechanism of NHase enzymes will enable access to nitrile-hydrolyzing materials with broader substrate ranges, higher activities, and greater stabilities. These biomaterials will also provide a "green" alternative to the harsh industrial conditions required for nitrile hydration.

这项《生命过程化学过程的合作研究奖》（CLP）计划支持芝加哥洛约拉大学化学和生物化学系的理查德·C·霍尔兹（Richard C. Holz）教授的工作，以进行有关铁和钴型硝酸盐水合物的催化机制的基本研究（CHE-1058396）。 NHASE以化学，区域和/或对映选择性方式催化了硝酸盐的水合为相应的商业有价值的酰胺，以环境压力，温度和生理pH值。 由于这些原因，NHases在制备有机化学和含细菌的生物催化剂中引起了很大的兴趣范围，更高的活动和更大的稳定性。 这些生物材料还将为氮化水分所需的严酷工业条件提供“绿色”替代品。