RAPID: ACQUISITION OF A PROTEOMICS ANALYZER TO ELUCIDATE PATHWAYS OF PETROLEUM HYDROCARBON BIOREMEDIATION IN THE GULF OF MEXICO

RAPID：购买蛋白质组分析仪来阐明墨西哥湾石油碳氢化合物生物修复的途径

• 批准号: 1057414
• 负责人: Kartik Chandran
• 金额: $ 20万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1057414&HistoricalAwards=false
• 关键词: RAPID; ACQUISITION; PROTEOMICS; ANALYZER; ELUCIDATE

PI: Kartik ChandranProposal Number: 1057414Institution: Columbia UniversityTitle: OIA: Acquisition of a Proteomics Analyzer to Elucidate Pathways of Petroleum Hydrocarbon Bioremediation in the Gulf of MexicoThis NSF MRI RAPID project will support the acquisition of a state-of-the art instrument to elucidate the microbial mechanisms of methane and complex petroleum hydrocarbon biodegradation at the quantitative proteomics level. The mechanistic information thus obtained will be used to develop bioremediation strategies to alleviate the environmental insults that have resulted from the Deepwater Horizon oil spill in the Gulf of Mexico. The Deepwater Horizon oil spill has resulted in drastically impaired aquatic life and environmental health of the gulf. Nevertheless, the specific chemical composition of the contaminant stream presents a novel opportunity for simultaneous bioremediation of both the primary organics (the petroleum hydrocarbons) and the methane (the purported cause of the explosion that led to the spill). Methane is typically the primary energy substrate for methane oxidizing bacteria (MOB). However, owing to the broad substrate specificity of the first enzyme involved in methane oxidation to formaldehyde (methane monooxygenase), MOB can oxidize not only methane but also alternate organic substrates such as longer chain aliphatic and aromatic compounds. However, MOB cannot derive energy from such transformations, which are termed ?co-metabolic?. Co-metabolism based bioremediation strategies are therefore characterized by a finite transformation capacity, limited by availability of the primary substrate (in casu, methane). In this project, they will elucidate the proteomics scale mechanisms of MOB to oxidize methane (via primary energy metabolism) and the mix of petroleum hydrocarbons (via co-metabolism) in lab-scale bioreactors. This NSF MRI Rapid project will support the acquisition of a quantitative proteomics analyzer (Waters Corp.), which will be housed initially for six-months in Earth and Environmental Engineering (the home department of the PI, Dr. Kartik Chandran) and then deployed onsite in the Gulf. When used for ex-situ bioremediation of the sequestered or skimmed pollutants along with the methane (which is currently just being flared or released), the bio-process technology developed in this project could be specifically applied to address both contaminants simultaneously. Mathematical models that describe methane oxidation and co-metabolism of petroleum hydrocarbons will be constructed and parameterized using the proteomics data combined with chemical profiles and microbial measurements. Finally, operating strategies, ex-situ transformation and mineralization of methane and petroleum hydrocarbons mixes obtained on site will be developed and tested. Successful application of this project will enable the accelerated biological treatment of the widespread petroleum hydrocarbon and methane pollution in the Gulf of Mexico. Additionally, the developed strategies will help to accelerate treatment and minimize such widespread dissemination of this particular contaminant mix, which is typical of offshore drilling operations in the future.This instrument will be used for Gulf oil spill related research in a timely fashion consistent with RAPID funding requirements.

PI: Kartik ChandranProposal Number: 1057414Institution: Columbia UniversityTitle: OIA: Acquisition of a Proteomics Analyzer to Elucidate Pathways of Petroleum Hydrocarbon Bioremediation in the Gulf of MexicoThis NSF MRI RAPID project will support the acquisition of a state-of-the art instrument to elucidate the microbial mechanisms of methane and complex petroleum定量蛋白质组学水平的烃生物降解。因此获得的机械信息将用于制定生物修复策略，以减轻墨西哥湾深水地平线漏油所造成的环境侮辱。深水地平线漏油事件导致海湾的水生寿命和环境健康造成了巨大损害。然而，污染物流的特定化学组成为同时生物修复主要有机物（石油碳氢化合物）和甲烷（所谓爆炸导致溢出的爆炸的原因）提供了新的机会。甲烷通常是用于甲烷氧化细菌（MOB）的主要能量底物。然而，由于与甲烷氧化的第一种酶的宽底物特异性（甲烷单加氧酶）相关，因此BOB不仅可以氧化甲烷，还可以氧化较长的有机底物，例如较长的链脂肪抗性和芳香族化合物。但是，暴民无法从这种转化中得出能量，这些转化是合作代谢的？因此，基于合法代谢的生物修复策略的特征是有限转化能力，受主要基质的可用性（在CASU，甲烷中）的限制。在这个项目中，他们将阐明BOB的蛋白质组学量表机制（通过一级能量代谢）和实验室生物反应器中石油烃（通过共生代谢）的混合物。该NSF MRI快速项目将支持对定量蛋白质组学分析仪（Waters Corp.）的获取，该项目最初将在地球和环境工程中为六个月（PI的家庭部门，Kartik Chandran博士）安置，然后在Gulf中部署现场。 当用于对隔离或脱脂污染物以及甲烷（目前刚刚爆发或释放）的隔离或脱脂污染物的前生物修复时，该项目中开发的生物过程技术可专门用于同时解决这两种污染物。描述石油烃的甲烷氧化和合作代谢的数学模型将使用蛋白质组学数据与化学剖面和微生物测量相结合来构建和参数化。最后，将开发和测试在现场获得的甲烷和石油碳氢化合物混合物的操作策略，前坐骨转化和矿化。该项目的成功应用将使墨西哥湾的广泛石油碳氢化合物和甲烷污染的加速生物处理能够加速。此外，开发的策略将有助于加速治疗，并最大程度地减少这种特殊污染物混合物的广泛传播，这将来是离岸钻探操作的典型特征。该仪器将用于及时符合快速资金要求的及时时尚相关研究。