Collaborative Research: Metabolic Rates and Growth Efficiency Across Redox and Thermal Gradients: An Experimental Study to Constrain Biomass Production at Vents

合作研究：氧化还原和热梯度下的代谢率和生长效率：限制通风口生物质生产的实验研究

基本信息

批准号：
1038131
负责人：
Stefan Sievert
金额：
$ 26.8万
依托单位：
Woods Hole Oceanographic Institution
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-15 至 2014-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1038131&HistoricalAwards=false
关键词：
Collaborative Research Metabolic Rates Growth

项目摘要

Although they are the base of the food-web in deep sea hydrothermal systems, the growth efficiencies and other essential metabolic characteristics of microorganisms that live off chemical energy from geothermal/mineralogical sources in hydrothermal vent areas in the deep ocean are poorly known. This research uses a novel, flow through, laboratory reactor apparatus to run first-of-a-kind experiments looking at microbial growth rates and metabolic efficiencies at the temperatures, pressures (100 to 250 bars), and chemical conditions found in hydrothermal systems on the deep seafloor. Experiments will target the metabolic processes of two common deep sea vent microorganisms by measuring, in the reactor solutions, nitrogen-bearing respiratory products of nitrate-reducing and hydrogen-oxidizing microbes. Research goals are to determine the role of redox gradients on nitrate reduction and ammonification in hydrothermal systems, use isotopes to determine metabolic rates using kinetic isotope effects, and determine the growth efficiency of mesophilic and thermophilic anaerobic microbes at a range of H2/CO2 molar ratios. Broader impacts of the work include cross-training of a graduate student in two disciplines and laboratories and inclusion of a student from an under-represented minority in research.

尽管它们是深海热液系统中食物网的基础，但以深海热液喷口区域地热/矿物来源的化学能为生的微生物的生长效率和其他基本代谢特征却鲜为人知。这项研究使用一种新颖的流通式实验室反应器装置来运行首个实验，观察在水热系统中发现的温度、压力（100 至 250 巴）和化学条件下的微生物生长速率和代谢效率。深海底。实验将通过测量反应器溶液中硝酸盐还原和氢氧化微生物的含氮呼吸产物来针对两种常见的深海喷口微生物的代谢过程。研究目标是确定氧化还原梯度对热液系统中硝酸盐还原和氨化的作用，利用同位素利用动力学同位素效应确定代谢率，并确定中温和嗜热厌氧微生物在一定范围的 H2/CO2 摩尔比下的生长效率。这项工作的更广泛影响包括对两个学科和实验室的研究生进行交叉培训，以及将来自代表性不足的少数群体的学生纳入研究中。