Collaborative Research: IDBR: Nanopore optical biosensor development for analyzing membrane protein interactions

合作研究：IDBR：用于分析膜蛋白相互作用的纳米孔光学生物传感器开发

• 批准号: 0964216
• 负责人: Sang-Hyun Oh
• 金额: $ 25万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0964216&HistoricalAwards=false
• 关键词: Collaborative; Research; IDBR; Nanopore; optical

Membrane proteins are one of the most important classes of molecules, representing over half of drug targets, and also one of the most poorly understood classes. This award is to leverage new technologies in nanofabrication, nano-optics, microfluidics and protein engineering to develop a nanopore membrane biosensor, which has the potential to transform membrane protein research. This real-time imaging instrument will exploit periodic nanopores patterned in a thin gold film to measure the binding kinetics and affinities between membrane-bound receptors and ligands - a task that is highly desirable but is rarely achieved with existing affinity biosensors such as BIAcoreTM. A thin gold film perforated with periodic nanopores will concurrently act as a mechanical scaffold to support cell membranes as well as a label-free surface plasmon resonance (SPR) sensor. Binding of ligands to immobilized membrane receptors will sharply modulate the light transmission through metallic nanopores, which is recorded as a real-time "movie" with high resolution. The system will be integrated with microfluidics to reduce sample consumption and enable multiplexing. While this instrument will be applicable to analyze potentially any membrane protein in a near-native lipid bilayer environment, initial studies will be performed with the key recognition events controlling cellular immunity. The ability to perform ligand screening for membrane receptors at high throughput and high resolution would present a breakthrough in studying these molecules and the fundamental life processes they enable.The integration of supported and free-standing lipid membranes with nanopore SPR sensors will provide an entirely novel approach for dynamic probing of membrane protein interactions, rather than adding incremental improvements to existing SPR instruments. To encourage adoption of the technology by biological groups, a two-day short course at the University of Minnesota (http://www.nano.umn.edu/biomems09/) will be expanded to include nanopore array fabrication, while the software and fabrication protocols will be freely available on the research website. To excite underrepresented groups about STEM research, SPR and protein-protein binding experiments will be incorporated into a training plan spanning middle-school through graduate students, placing special emphasis on undergraduates.

膜蛋白是最重要的分子类别之一，代表了一半以上的药物靶点，也是人们最了解的类别之一。该奖项旨在利用纳米制造、纳米光学、微流体和蛋白质工程方面的新技术来开发纳米孔膜生物传感器，该传感器具有改变膜蛋白研究的潜力。这种实时成像仪器将利用金薄膜上的周期性纳米孔来测量膜结合受体和配体之间的结合动力学和亲和力——这是一项非常理想的任务，但现有的亲和生物传感器（如BIAcoreTM）很少能实现这一任务。带有周期性纳米孔的金薄膜将同时充当支撑细胞膜的机械支架以及无标记的表面等离子共振（SPR）传感器。配体与固定膜受体的结合将急剧调节通过金属纳米孔的光传输，这被记录为高分辨率的实时“电影”。该系统将与微流体集成，以减少样品消耗并实现多重分析。虽然该仪器将适用于分析近天然脂质双层环境中的任何膜蛋白，但初步研究将针对控制细胞免疫的关键识别事件进行。以高通量和高分辨率对膜受体进行配体筛选的能力将为研究这些分子及其所支持的基本生命过程带来突破。支撑和独立式脂质膜与纳米孔 SPR 传感器的集成将提供一种全新的方法。动态探测膜蛋白相互作用的方法，而不是对现有 SPR 仪器进行增量改进。为了鼓励生物群体采用该技术，明尼苏达大学 (http://www.nano.umn.edu/biomems09/) 为期两天的短期课程将扩大到包括纳米孔阵列制造，同时软件和制造协议将在研究网站上免费提供。为了激发代表性不足的群体对 STEM 研究的兴趣，SPR 和蛋白质-蛋白质结合实验将被纳入涵盖初中生到研究生的培训计划中，并特别关注本科生。