MRI-R2 Consortium: Development of an 8kx8k pixel direct detection CMOS camera with single electron counting for cryoEM

MRI-R2 联盟：开发用于冷冻电镜的具有单电子计数功能的 8kx8k 像素直接检测 CMOS 相机

• 批准号: 0960271
• 负责人: David Agard
• 金额: $ 197.68万
• 依托单位: University of California-San Francisco
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0960271&HistoricalAwards=false
• 关键词: MRI; R2; Consortium; Development; 8kx8k; MRI; R2; Consortium; Development; 8kx8k

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the development of an 8kx8k pixel direct detection CMOS camera with single electron quantized detection for high-resolution single particle cryo electron microscopy (cryoEM) at the University of California-San Francisco (UCSF). The new camera system will enable high frame readout rates, with extreme sensitivity and high resolution. Because it uses so little material and does not require crystals, single particle cryoEM has become an indispensable tool for studying the three-dimensional (3D) structures of complex biological assemblies, providing critical information not obtainable by more traditional methods such as x-ray crystallography or NMR spectroscopy. The camera development project will be coordinated through collaborations among UCSF, the Lawrence Berkeley National Laboratory (LBNL), and Gatan, Inc., a company specializing in EM cameras and peripherals. The camera will benefit a large number of research projects and improve the training prospects for 15-25 students and postdoctoral fellows in advanced cryoEM technology. UCSF PIs are also active participants in summer research programs and have been hosting undergraduates in their labs to provide research opportunities with advanced technologies. Performance and availability of the camera will be widely disseminated through a web site, in publications, and through presentations at local and international meetings. Commercialization of the new technology may lead to even wider dissemination of similar devices.

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the development of an 8kx8k pixel direct detection CMOS camera with single electron quantized detection for high-resolution single particle cryo electron microscopy (cryoEM) at the University of California-San Francisco (UCSF).新的相机系统将具有高度灵敏度和高分辨率的高框架读数率。 由于它使用的材料很少，并且不需要晶体，因此单个粒子冷冻已成为研究复杂生物组件的三维（3D）结构的必不可少的工具，从而提供了不可用X射线晶体学或NMR光谱等更传统方法获得的关键信息。 相机开发项目将通过UCSF，Lawrence Berkeley国家实验室（LBNL）和专门从事EM摄像机和外围设备的公司Gatan，Inc。进行协调。 该相机将使大量研究项目受益，并改善高级冷冻技术中15-25名学生和博士后研究员的培训前景。 UCSF PI也是夏季研究计划的积极参与者，并一直在实验室中托管本科生，以提供高级技术的研究机会。相机的性能和可用性将通过网站，出版物以及在本地和国际会议上的演讲中广泛传播。 新技术的商业化可能会导致更广泛地传播类似设备。