Collaborative Research: MSB: Defining Plant-Associated Metagenomics

合作研究：MSB：定义植物相关宏基因组学

• 批准号: 0958245
• 负责人: Jeffery Dangl
• 金额: $ 249.02万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-15 至 2015-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0958245&HistoricalAwards=false
• 关键词: Collaborative; Research; MSB; Defining; Plant

All land plants grow in association with complex microbial communities both on aboveground plant organs (called the phyllosphere) and below the ground on roots (called therhizosphere). The relationships between a microbiota (the community of microbesintimately associated with a plant) and its host can vary from pathogenic to symbiotic.The microbiome (the set of genes encoded by any particular microbiota) can performecosystem services by providing the host plant with critical nutrients, protection frompathogens, production of functional plant hormones, and provision of tolerance tostressors like heat. The plant, in turn, provides the microbiome with an improvedenvironment, including a complex array of energy sources released by deposition ofplant-based sugars into the soil. Decaying plant tissue and carbon-rich root exudatesredirect large amounts of fixed atmospheric carbon to the soil, making the rhizosphere alarge carbon sequestration zone, important in the balance of carbon in a changingenvironment. Site specific characteristics of climate and soil that influence the yield ofmaize and other crops and the productivity of any plant community rely in part on therespective rhizosphere microbiome. This research will ultimately lead to agriculturalpractices designed to increase plant health and productivity, carbon sequestration, anddisease resistance through the rational utilization of the probiotic capacity of soils andthe local environment. The project team is a collaborative interdisciplinary group fromUNC-Chapel Hill, Cornell and the DOE Joint Genome Institute. The experiments to becarried out will define the rhizosphere microbiota and associated microbiome using twoimportant model plant species: Arabidopsis thaliana, the premier model for rapiddiscovery in plant biology, and Zea mays, the premier model for genetic dissection ofcomplex traits in crops. Data generated by the project will be made freely available (http://www.bio.unc.edu/dangl/lab/projects/Metagenome_2-3-10.htm; http://www.ncbi.nlm.nih.gov/Genbank/), and will provide starting points for a variety of deeper studies into the complexities of host-microbe interactions. The project will provide training opportunities for undergraduates, doctoral students and post-doctoral fellows.

所有土地植物都与地上植物器官（称为植物圈）和根部（称为therhizosphere）的地面上的复杂微生物群落合并。微生物群（与植物的微生物群落）及其宿主之间的关系可能从致病性到共生。微生物组（任何特定的微生物群编码的基因）可以通过为宿主提供关键营养素，保护植物的生产，功能性植物和耐受性的生产，可通过为宿主提供宿主的植物来表现服务系统服务，并提供耐受性。该植物反过来为微生物组提供了改善的环境，其中包括由基于植物的糖沉积到土壤中释放的一系列能源。腐烂的植物组织和富含碳的根部散发出大量的固定大气碳到土壤中，从而使根际含量碳固隔区对碳平衡很重要。影响气候和土壤的特定特征，这些特征会影响土壤和其他农作物的产量以及任何植物群落的生产力，部分依赖于根际小变根微生物组。这项研究最终将导致农业实践，旨在通过合理利用土壤和当地环境的益生菌能力来提高植物健康和生产力，碳固执和抗药性。项目团队是一个合作的跨学科小组Forthunc-Chapel Hill，Cornell和DOE联合基因组学院。进行审查的实验将使用两种可能的模型植物物种来定义根际微生物群和相关的微生物组：拟南芥，植物生物学中快速分散的主要模型，Zea Mays，Zea Mays，这是Comcomplex特征的遗传性剖析模型。该项目生成的数据将免费提供（http://www.bio.unc.edu/dangl/lab/lab/projects/metagenome_2-3-10.htm; http：//www.ncbi.nlmm.nlm.nlm.nih.gov/genbank/将为M-MONTOBE提供了多样的研究，并将提供多种研究，并将提供各种研究。该项目将为大学生，博士生和博士后研究员提供培训机会。