EAGER: Studies of native grassland soil nematodes to develop genomic approaches to study community responses in non-model taxa

EAGER：对本土草原土壤线虫的研究，开发基因组方法来研究非模型类群的群落反应

• 批准号: 0956614
• 负责人: Michael Herman
• 金额: $ 4万
• 依托单位: Kansas State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0956614&HistoricalAwards=false
• 关键词: EAGER; Studies; native; grassland; soil; EAGER; Studies; native; grassland; soil

The community of bacteria and microorganisms in the soil is fundamental to ecosystem processing such a nutrient cycling, but has been hard to study in the past because the organisms are so small. New technology in gene and genome sequencing now permits detailed analyses of the sorts of genetic changes that occur in microorganisms in response to human disturbance and climate change. This project will investigate four species of soil nematodes, tiny worms that eat bacteria, native to the Konza Prairie in Kansas. Each species of nematode will be grown on each of four native species of bacteria to determine which genes are most strongly expressed in the worms when they occur with each of the bacteria. The data can be compared to detailed experimental data already available for the well-studied model nematode worm species, Caenorhabditis elegans.By learning how particular nematodes respond to the bacteria that they contact and consume, it will be possible to develop better models of how the soil community responds to disturbance in both the short term and the long term. We are only beginning to learn which soil bacteria, nematodes, and other microorganisms are beneficial or harmful in particular situations, and what their role is in helping to stabilize plant and animal communities. This project will provide a uniquely detailed view of the interactions that occur among the tiny denizens of the soil and will enhance scientific infrastructure through provision of genetic data and analytical tools.

土壤中细菌和微生物的群落是生态系统加工这种营养循环的基础，但过去很难研究，因为这些生物很小。 现在，基因和基因组测序中的新技术允许详细分析微生物在响应人类疾病和气候变化时发生的遗传变化。 该项目将调查四种土壤线虫，即堪萨斯州Konza Prairie原产的细菌的微小蠕虫。 每种细菌的本地种类中的每一种都会生长每种线虫，以确定每种细菌发生在蠕虫中最强烈表达的基因。 可以将数据与已经可用于良好模型线虫蠕虫秀丽隐杆线虫的详细实验数据进行比较。通过了解特定的线虫对它们接触和消费的细菌的反应如何，可以发展出更好的模型，以更好地了解短期和长期对土壤社区的反应。 我们才开始了解哪种土壤细菌，线虫和其他微生物在特定情况下是有益或有害的，以及它们在帮助稳定动植物社区方面的作用。 该项目将提供对土壤微小居民之间相互作用的独特详细观点，并通过提供遗传数据和分析工具来增强科学基础设施。