RAMAN MICROSCOPE FOR PROBING NANO-BIO INTERFACES AND COMPLEX SYSTEMS

用于探测纳米生物界面和复杂系统的拉曼显微镜

• 批准号: 0933621
• 负责人: Ponisseril Somasundaran
• 金额: $ 7万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0933621&HistoricalAwards=false
• 关键词: RAMAN; MICROSCOPE; PROBING; NANO; BIO

0933621SomasundaranThis project is for the acquisition of an analytical grade confocal Raman microscope for the UFL/CU I/UCRC NSF Center for Particulate and Surfactant Systems (CPSS) at Columbia University, for a shared use with other departments. The instrument is essential in advancing current and planned research at CPSS in such areas as 1) health and environmental safety and chemical reactivity of nanoparticles (NPs); 2) greener chemicals for industries; 3) new silicone surfactants and polymers with enhanced performance. Among current projects in the Department of Earth and Environment Engineering and other departments which would also definitely benefit from the use of Raman are: 1) carbon dioxide sequestration, alternative energy, gas separation membranes; 2) biocolloid engineering for medical applications in molecular imaging and targeted therapy; 3) determination of geological processes through identification of dissolved volatiles in high silica magmas, identification of mineral phases in multi-micron sized inclusions within host crystals; 4) new nanoelectronical systems; unique electronic, rotational, vibrational and spin interactions between an incarcerated guest molecules with the inner concave walls of a C60 host.Intellectual Merit: 1) Unique, fast, accurate, and non-intrusive techniques of Raman spectroscopy will be developed for in situ locating of NPs and characterizing of their electronic, surface, and lattice properties, wettability, agglomeration, and deposition as well as reactivity (adsorption, acid-base, redox properties, and the capacity of NPs to generate or scavenge reactive oxygen species, interaction with cell membranes) at natural and engineered interfaces as well as in cells, bacteria, emulsions, and suspensions. 2) Based on the scientific data obtained through Raman and other complementary spectroscopic and microscopic methods, a new paradigm of nanotoxicity of NPs will be developed. 3) In combination with other experimental methods and theoretical modeling, Raman microscopy will be used to understand the synergistic effects of green surfactants with conventional surfactants, polymers and their hybrids at molecular and upramolecular levels. Fundamental mechanisms and predictive models for the aggregation behavior and structure-performance relationship will be proposed and tested. 4) For the first time, a systematic fundamental vibrational spectroscopic study will be performed on the surface and interfacial activity of silicone surfactants/polymers on cotton fabric, as a function of silicone molecular architecture. The information generated will be used to expand the existing knowledge base to develop new hybrid silicone structures and to identify other materials with unique interfacial properties.Broader Impact: The use of Raman microscopy in our research efforts will allow 1) the generation of critical knowledge required to address challenges in the emerging fields of green technologies, nanotechnology and nanoscience, colloid science, geoscience, medicine, and materials research; 2) the development of new technological solutions based on a better understanding of novel and complex materials and their interactions with biosystems at the molecular level, in particular, fostering innovation in a broad range of industries that employ surfactants, polymers, and nanoparticles; 3) the enhancement of interdisciplinary and inter departmental collaboration; 4) the training of undergraduate and graduate students on an instrument with utility across a wide range of disciplines, promoting their education and their team interaction; 5) the expansion of the scope of the spectrochemistry community into the emerging areas of applied sciences and engineering, which will certainly boost new high quality interdisciplinary research. The research will help to increase the number of minority students in various laboratories, as we are aiming to train more minority students through the Engineering school's special Undergraduate Research Involvement program. Minority students will benefit through first hand experience in methods of scientific research, working with advanced analytical techniques, and learning about their applications in industrially relevant areas. The PI will integrate a part of the employed experimental studies to develop a laboratory module for use in his courses Introduction to Surface and Colloid Chemistry and Applied Surface and Colloid Chemistry.

0933621Somasundaran该项目旨在为哥伦比亚大学 UFL/CU I/UCRC NSF 颗粒和表面活性剂系统中心 (CPSS) 购买一台分析级共焦拉曼显微镜，以便与其他部门共享。该仪器对于推进 CPSS 当前和计划中的以下领域的研究至关重要：1) 健康和环境安全以及纳米颗粒 (NP) 的化学反应性； 2）更环保的工业化学品； 3）新型有机硅表面活性剂和性能增强的聚合物。地球与环境工程系和其他部门目前的项目也肯定会从拉曼的使用中受益，其中包括：1）二氧化碳封存、替代能源、气体分离膜； 2）生物胶体工程在分子成像和靶向治疗中的医学应用； 3）通过鉴定高硅岩浆中溶解的挥发物、鉴定主晶体内多微米大小的包裹体中的矿物相来确定地质过程； 4）新型纳米电子系统；被监禁的客体分子与 C60 主体的内凹壁之间独特的电子、旋转、振动和自旋相互作用。智力优点：1）将开发独特、快速、准确和非侵入性的拉曼光谱技术用于原位定位纳米粒子及其电子、表面和晶格特性、润湿性、团聚和沉积以及反应性（吸附、酸碱、氧化还原特性和容量）的表征纳米粒子在自然和工程界面以及细胞、细菌、乳液和悬浮液中产生或清除活性氧（与细胞膜相互作用）。 2）基于通过拉曼和其他补充光谱和显微方法获得的科学数据，将开发纳米颗粒纳米毒性的新范式。 3）结合其他实验方法和理论模型，拉曼显微镜将用于了解绿色表面活性剂与常规表面活性剂、聚合物及其杂化物在分子和超分子水平上的协同效应。将提出并测试聚合行为和结构-性能关系的基本机制和预测模型。 4) 首次对棉织物上有机硅表面活性剂/聚合物的表面和界面活性进行系统的基础振动光谱研究，作为有机硅分子结构的函数。生成的信息将用于扩展现有知识库，以开发新的混合有机硅结构并识别具有独特界面特性的其他材料。更广泛的影响：在我们的研究工作中使用拉曼显微镜将允许 1) 生成所需的关键知识应对绿色技术、纳米技术和纳米科学、胶体科学、地球科学、医学和材料研究等新兴领域的挑战； 2）基于对新颖和复杂材料及其在分子水平上与生物系统的相互作用的更好理解，开发新技术解决方案，特别是促进使用表面活性剂、聚合物和纳米粒子的广泛行业的创新； 3）加强跨学科、跨部门的协作； 4) 对本科生和研究生进行跨学科实用仪器的培训，促进他们的教育和团队互动； 5）将光谱化学界的范围扩展到应用科学和工程的新兴领域，这必将促进新的高质量跨学科研究。这项研究将有助于增加各个实验室的少数族裔学生数量，因为我们的目标是通过工程学院的特殊本科生研究参与计划培养更多的少数族裔学生。少数族裔学生将通过科学研究方法的第一手经验、使用先进的分析技术以及了解其在工业相关领域的应用而受益。 PI 将整合部分所采用的实验研究来开发一个实验室模块，用于他的课程“表面和胶体化学简介”以及“应用表面和胶体化学”。