Chitosan Fiberbased Threedimensional Hybrid Scaffolds for Bone Tissue Engineering

用于骨组织工程的基于壳聚糖纤维的三维混合支架

基本信息

批准号：
191760137
负责人：
Professor Dr.-Ing. Chokri Cherif
金额：
--
依托单位：
Max Bergmann Zentrum für Biomaterialien
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2011
资助国家：
德国
起止时间：
2010-12-31 至 2016-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/191760137?language=en
关键词：
Chitosan Fiberbased Threedimensional Hybrid Scaffolds

项目摘要

Novel NSN-Hybrid-Scaffolds made of chitosan micro- and nanofibres which are constructed in hierarchically simple scaffold structures with defined porosity, were successfully developed in the first stage of funding. Their suitability as a 3D-scaffold structure for bone TE was tested in vitro. Based on the extensive and promising results of the first stage of funding novel, complexly structured and functionalized NSN-Hybrid-Scaffolds with local adjustable porosities are to be developed in the proposed second stage of funding, in order to generate a scaffold, which has the same structure and material composition as the bone tissue. Therefore, the main objective is the development of textile technological solutions for the realization of such complex collagen-coated NSN-Hybrid-Scaffolds with local adjustable pore gradients and both numerically definable and reproducible properties. For this purpose, the properties of the complex NSN structures shall be modelled by means of computer systems in order to predict the relationships between the applied fibre types, fabrication parameters and coatings and the thus resulting structure and porosity as well as to drive the design guidelines for NSN-Hybrid-Scaffolds. A continuous process chain that uses the algorithms developed in the material model must be established, allowing the defined and pre-set fabrication of complexly structured NSN-Hybridscaffolds and thus minimizing trial & error attempts. A new quantitative method must be developed to analyse the pore size gradients in a statistically valid way. The application of collagen coating for the generation of pore gradients, which is also advantageous for cell response, requires a further study of the influential parameters in the coating of NSN-Hybridscaffolds. A further improvement in cell adhesion, proliferation and differentiation is intended by the targeted mineralization of NSN-Hybrid-Scaffolds and functionalization with growth factors (BMP-2, or VEGF) on the nanofibre-scale. The absorption kinetics of the NSN-Scaffolds will be investigated by varying the fibre types and sterilization methods. By means of fluorescence labelling of the chitosan fibres within the NSN-Hybrid-Scaffolds the bioresorption will be studied in the presence of bone resorbing osteoclasts to obtain conclusions regarding the in vivo degradation behaviour. The most suitable scaffold functionalization for bone cells will be determined by cell culture experiments in mono- and co-cultures. In preparation for in vivo studies the influence of the NSN-Scaffolds on the formation of blood vessels will be examined. As a result of this research work a NSN model scaffold shall be provided, which seems promising for future in vivo experiments.

由壳聚糖微米纤维和纳米纤维制成的新型 NSN 混合支架在第一阶段融资中已成功开发，该支架结构具有分层简单的支架结构，具有明确的孔隙率。它们作为骨 TE 的 3D 支架结构的适用性在体外进行了测试。基于第一阶段资金的广泛和有希望的结果，在拟议的第二阶段资金中将开发具有局部可调节孔隙率的新型、复杂结构和功能化的 NSN 混合支架，以生成一个支架，该支架具有与骨组织相同的结构和材料成分。因此，主要目标是开发纺织技术解决方案，以实现这种复杂的胶原蛋白涂层 NSN 混合支架，其具有局部可调节的孔隙梯度以及数值可定义和可重复的特性。为此，应通过计算机系统对复杂 NSN 结构的特性进行建模，以预测所应用的光纤类型、制造参数和涂层之间的关系以及由此产生的结构和孔隙率，并推动设计指南适用于 NSN 混合支架。必须建立使用材料模型中开发的算法的连续工艺链，允许定义和预设制造复杂结构的 NSN-Hybridscaffolds，从而最大限度地减少试错尝试。必须开发一种新的定量方法以统计上有效的方式分析孔径梯度。应用胶原蛋白涂层产生孔隙梯度，这也有利于细胞反应，需要进一步研究 NSN-Hybridscaffolds 涂层中的影响参数。通过 NSN-Hybrid-Scaffolds 的靶向矿化和纳米纤维规模的生长因子（BMP-2 或 VEGF）功能化，可进一步改善细胞粘附、增殖和分化。 NSN-Scaffolds 的吸收动力学将通过改变纤维类型和灭菌方法进行研究。通过对 NSN-Hybrid-Scaffold 内的壳聚糖纤维进行荧光标记，将在骨吸收破骨细胞存在的情况下研究生物吸收，以获得有关体内降解行为的结论。最适合骨细胞的支架功能化将通过单一培养和共培养的细胞培养实验来确定。在准备体内研究时，将检查 NSN 支架对血管形成的影响。这项研究工作的结果将提供一个 NSN 模型支架，这对于未来的体内实验来说似乎很有希望。