Chitosan Fiberbased Threedimensional Hybrid Scaffolds for Bone Tissue Engineering

用于骨组织工程的基于壳聚糖纤维的三维混合支架

基本信息

批准号：
191760137
负责人：
Professor Dr.-Ing. Chokri Cherif
金额：
--
依托单位：
Max Bergmann Zentrum für Biomaterialien
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2011
资助国家：
德国
起止时间：
2010-12-31 至 2016-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/191760137?language=en
关键词：
Chitosan Fiberbased Threedimensional Hybrid Scaffolds

项目摘要

Novel NSN-Hybrid-Scaffolds made of chitosan micro- and nanofibres which are constructed in hierarchically simple scaffold structures with defined porosity, were successfully developed in the first stage of funding. Their suitability as a 3D-scaffold structure for bone TE was tested in vitro. Based on the extensive and promising results of the first stage of funding novel, complexly structured and functionalized NSN-Hybrid-Scaffolds with local adjustable porosities are to be developed in the proposed second stage of funding, in order to generate a scaffold, which has the same structure and material composition as the bone tissue. Therefore, the main objective is the development of textile technological solutions for the realization of such complex collagen-coated NSN-Hybrid-Scaffolds with local adjustable pore gradients and both numerically definable and reproducible properties. For this purpose, the properties of the complex NSN structures shall be modelled by means of computer systems in order to predict the relationships between the applied fibre types, fabrication parameters and coatings and the thus resulting structure and porosity as well as to drive the design guidelines for NSN-Hybrid-Scaffolds. A continuous process chain that uses the algorithms developed in the material model must be established, allowing the defined and pre-set fabrication of complexly structured NSN-Hybridscaffolds and thus minimizing trial & error attempts. A new quantitative method must be developed to analyse the pore size gradients in a statistically valid way. The application of collagen coating for the generation of pore gradients, which is also advantageous for cell response, requires a further study of the influential parameters in the coating of NSN-Hybridscaffolds. A further improvement in cell adhesion, proliferation and differentiation is intended by the targeted mineralization of NSN-Hybrid-Scaffolds and functionalization with growth factors (BMP-2, or VEGF) on the nanofibre-scale. The absorption kinetics of the NSN-Scaffolds will be investigated by varying the fibre types and sterilization methods. By means of fluorescence labelling of the chitosan fibres within the NSN-Hybrid-Scaffolds the bioresorption will be studied in the presence of bone resorbing osteoclasts to obtain conclusions regarding the in vivo degradation behaviour. The most suitable scaffold functionalization for bone cells will be determined by cell culture experiments in mono- and co-cultures. In preparation for in vivo studies the influence of the NSN-Scaffolds on the formation of blood vessels will be examined. As a result of this research work a NSN model scaffold shall be provided, which seems promising for future in vivo experiments.

由壳聚糖微纤维和纳米纤维制成的新型NSN杂交型夫人在层次上简单的脚手架结构中构建具有定义的孔隙率，在资金的第一阶段成功地开发了。在体外测试了它们作为骨头TE的3D支线结构的适用性。基于资助小说的第一阶段的广泛而有希望的结果，在拟议的第二阶段的资助第二阶段将开发出具有局部可调孔隙性的复杂结构化和功能化的NSN杂交型，以产生脚手架，以产生脚手架，该支架具有与骨骼组织相同的结构和材料组成。因此，主要目的是开发纺织技术解决方案，以实现具有局部可调孔梯度以及数值定义和可重复可重复的特性，以实现这种复杂的胶原蛋白包被的NSN Hybrid-Scagfolds。为此，应通过计算机系统对复杂的NSN结构的特性进行建模，以预测所施加的纤维类型，制造参数和涂料与因此产生的结构和孔隙率以及驱动NSN-Hybrid-Scaffolds的设计准则。必须建立使用材料模型中开发的算法的连续过程链，从而允许定义并预先设置复杂结构的NSN-HYBRIDSCAFFOLDS，从而最大程度地减少试用和错误尝试。必须开发一种新的定量方法来以统计有效的方式分析孔径梯度。胶原蛋白在生成孔隙梯度的生成中的应用，这对于细胞反应也有利，需要进一步研究NSN-HybridScaffolds涂层中的影响参数。细胞粘附，增殖和分化的进一步改善旨在通过纳米纤维尺度上的NSN杂交型和生长因子（BMP-2或VEGF）的靶向矿化和生长因子（BMP-2或VEGF）的功能化。 NSN-Scakolds的吸收动力学将通过改变纤维类型和灭菌方法来研究。通过NSN杂交型造成的壳聚糖纤维的荧光标记，将在存在骨骨分辨破骨细胞的情况下研究生物吸附，以获得有关体内降解行为的结论。骨细胞最合适的支架功能化将由单培养和共培养中的细胞培养实验确定。为了准备体内研究，将检查NSN-SCASCADGOLDS对血管形成的影响。作为这项研究工作的结果，应提供NSN模型支架，这对于未来的体内实验似乎很有希望。