Directed Evolution of Specific Cell Penetrating Peptides

特定细胞穿透肽的定向进化

• 批准号: 0853946
• 负责人: Scott Banta
• 金额: $ 30.17万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0853946&HistoricalAwards=false
• 关键词: Directed; Evolution; Specific; Cell; Penetrating

This award is funded under the American Recovery and Reinvestment Act of 2009(Public Law 111-5).0853946Banta Intellectual merit The intellectual merit of this proposal results from the first demonstration of the directed evolution of specific cell penetrating peptides (SCPPs). The directed evolution technique offers tremendous potential for the engineering of new peptides, but the critical challenge in this approach is the development of appropriate selection procedures. The PIs will use a novel bi-functional platform based on phage display technology, and this will be coupled with a physiologically relevant selection protocol. Once optimized, this platform will allow one to rapidly evolve valuable new SCPP sequences.Broader impacts The broader impacts of this project arise from the new experimental manipulations and therapeutic treatment options that will be made possible by the novel SCPPs. Recent research efforts have produced many exciting mechanism-based therapies. However, the delivery of these therapies to their targets has been a significant limitation. The new SCPPs will enable the expansion of the armamentarium of potential therapies for these devastating diseases. This interdisciplinary research project will also provide a fertile environment for the teaching, training, and mentoring of new engineers both at Columbia University and in the surrounding community.

该奖项是根据2009年的《美国复苏与再投资法》（公法111-5）的资助。0853946Banta知识分子优胜这项提案的知识分子，这是由于首次证明了特定细胞穿透性肽（SCPP）的定向演变。定向的进化技术为新肽的工程提供了巨大的潜力，但是这种方法的关键挑战是开发适当的选择程序。 PI将使用基于噬菌体显示技术的新型双功能平台，并将与生理相关的选择方案结合使用。 一旦进行了优化，该平台将允许人们快速发展有价值的新SCPP序列。BRODER会影响该项目的更广泛影响，这是由新的SCPP将成为可能成为可能的新实验操作和治疗方法。最近的研究工作产生了许多基于机制的疗法。但是，将这些疗法递送到目标是一个重大限制。新的SCPP将使这些毁灭性疾病的潜在疗法的武器群扩展。该跨学科研究项目还将为哥伦比亚大学和周边社区的新工程师的教学，培训和指导提供一个肥沃的环境。