Regulation of the anaphase-promoting complex by pseudosubstrate inhibition

通过假底物抑制调节后期促进复合物

• 批准号: 0841748
• 负责人: Mark Hall
• 金额: $ 58.66万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0841748&HistoricalAwards=false
• 关键词: Regulation; anaphase; promoting; complex; pseudosubstrate

Research Project Description. The process of cell division, by which a single parent cell gives rise to two identical daughter cells, is amazingly complex. At its core are the duplication of the cell's entire genome and the accurate distribution of one intact copy of the genome to each of the two daughter cells. Remarkably, almost all non-bacterial species use the same basic machinery and regulatory controls to achieve cell division. How the complex steps involved in cell division are regulated is still only partially understood. In this project, budding yeast will be used as a simple model system to study one essential component of cell division control, regulated protein destruction. The project aims to understand how a key enzyme responsible for protein destruction during cell division is activated and inactivated, and the mechanism by which it specifically recognizes its targets. By using the yeast model system, a broad array of molecular techniques spanning the fields of cell biology, genetics, biochemistry, and biophysics will be applied to achieve the aims. The project will help advance the general understanding of molecular mechanisms of 1) cell division control, and 2) regulated protein destruction.Broader Impact. The research project is associated with graduate and undergraduate programs in biochemistry. A major focus is education and training of students in critical thinking and the scientific method, essential skills for future careers as independent research scientists and important skills for any science-related career. A secondary focus is development of research skills in a broad array of fundamental molecular biological techniques that will prepare students for a variety of research-related careers. Finally, a third goal is development of effective scientific communication skills. The culmination of this goal is presentation of research results at national scientific meetings by students and publication of research papers in peer-reviewed scientific journals. In summary, this project will help train the next generation of research scientists and contribute to the public collection of basic biological knowledge.

研究项目描述。细胞分裂的过程（单亲细胞产生两个相同的子细胞）的过程非常复杂。其核心是细胞整个基因组的重复以及一个基因组完整副本到两个子细胞中每个细胞中每个细胞的准确分布。值得注意的是，几乎所有非细菌物种都使用相同的基本机制和调节控制来实现细胞分裂。如何调节细胞分裂中涉及的复杂步骤仍然仅部分理解。在该项目中，萌芽酵母将被用作一个简单的模型系统，以研究细胞分裂控制的一个基本组成部分，即受调节的蛋白质破坏。该项目旨在了解如何激活和灭活细胞分裂过程中造成蛋白质破坏的关键酶以及其专门识别其靶标的机制。通过使用酵母模型系统，将应用一系列跨越细胞生物学，遗传学，生物化学和生物物理学领域的分子技术来实现目标。该项目将有助于提高对1）细胞分裂控制的分子机制的一般理解，以及2）受调节的蛋白质破坏。该研究项目与生物化学的研究生和本科课程有关。一个重点是对批判性思维和科学方法的学生的教育和培训，作为独立研究科学家的未来职业的重要技能以及与科学相关职业的重要技能。第二个重点是在广泛的基本分子生物学技术中发展研究技能，这将使学生为各种与研究相关的职业做好准备。最后，第三个目标是发展有效的科学沟通技巧。这个目标的结晶是在学生会在国家科学会议上介绍研究结果，并在同行评审的科学期刊上发表研究论文。总而言之，该项目将有助于培训下一代研究科学家，并为公众收集基本的生物学知识做出贡献。