Multiple biomarker sensor for early detection of ovarian cancer

用于早期检测卵巢癌的多生物标志物传感器

• 批准号: 0801929
• 负责人: Venkat Bhethanabotla
• 金额: $ 32.96万
• 依托单位: University of South Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0801929&HistoricalAwards=false
• 关键词: Multiple; biomarker; sensor; early; detection

ObjectiveThe objective of this research is to develop a sensor for the detection of multiple biomarkers with specific application to early detection and monitoring of ovarian cancer. The approach is based on a surface acoustic wave (SAW) transducer that integrates two critical functions, namely, removal of interfering proteins, and sensing of multiple biomarkers, on a single device. Technical MeritThis project is the first to propose and demonstrate removal of non-specifically bound (NSB) proteins from sensor surfaces using SAWs. NSB is a confounding factor for sensor response utilizing any sensing principle, optical, electrochemical or mass change. The proposed sensor is the first to integrate this removal function with the sensing function of any device. This is the first attempt at differential removal of bound proteins, one at a time, to achieve detection and quantification of multiple moieties on a single device surface. Many diseases, such as ovarian cancer, are characterized by elevated levels of select proteins in bodily fluids. Hence, this sensor will have wide applicability in medical diagnostics. Broader ImpactThis work will allow for more wide-spread and easy testing than current practice and is also applicable to detection of multiple moieties for environmental and chemical detection applications. The proposed pilot testing of an interdisciplinary senior design project can lead to a sustainable model for engineering education. Proposed hands-on sensor units and associated experiments will lead to awareness of basic sensor principles and concepts among the participating school children. The proposed minority mentoring program can lead to recruitment into science and engineering programs.

目的本研究的目的是开发一种用于检测多种生物标志物的传感器，专门应用于卵巢癌的早期检测和监测。 该方法基于表面声波 (SAW) 传感器，该传感器在单个设备上集成了两个关键功能，即去除干扰蛋白和感测多个生物标记物。技术优点该项目是第一个提出并证明使用 SAW 从传感器表面去除非特异性结合 (NSB) 蛋白质的项目。 NSB 是利用任何传感原理（光学、电化学或质量变化）的传感器响应的混杂因素。 所提出的传感器是第一个将这种去除功能与任何设备的传感功能集成在一起的传感器。 这是一次一次有差别地去除结合蛋白的第一次尝试，以实现单个设备表面上多个部分的检测和定量。 许多疾病，例如卵巢癌，其特征是体液中特定蛋白质的水平升高。因此，该传感器将在医疗诊断中具有广泛的应用性。 更广泛的影响这项工作将允许比当前实践更广泛和更容易的测试，并且还适用于环境和化学检测应用的多个部分的检测。 拟议的跨学科高级设计项目试点测试可以形成可持续的工程教育模式。 拟议的动手传感器单元和相关实验将使参与的学童了解基本的传感器原理和概念。 拟议的少数族裔指导计划可以导致科学和工程项目的招募。