Multiple biomarker sensor for early detection of ovarian cancer

用于早期检测卵巢癌的多生物标志物传感器

• 批准号: 0801929
• 负责人: Venkat Bhethanabotla
• 金额: $ 32.96万
• 依托单位: University of South Florida
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0801929&HistoricalAwards=false
• 关键词: Multiple; biomarker; sensor; early; detection

ObjectiveThe objective of this research is to develop a sensor for the detection of multiple biomarkers with specific application to early detection and monitoring of ovarian cancer. The approach is based on a surface acoustic wave (SAW) transducer that integrates two critical functions, namely, removal of interfering proteins, and sensing of multiple biomarkers, on a single device. Technical MeritThis project is the first to propose and demonstrate removal of non-specifically bound (NSB) proteins from sensor surfaces using SAWs. NSB is a confounding factor for sensor response utilizing any sensing principle, optical, electrochemical or mass change. The proposed sensor is the first to integrate this removal function with the sensing function of any device. This is the first attempt at differential removal of bound proteins, one at a time, to achieve detection and quantification of multiple moieties on a single device surface. Many diseases, such as ovarian cancer, are characterized by elevated levels of select proteins in bodily fluids. Hence, this sensor will have wide applicability in medical diagnostics. Broader ImpactThis work will allow for more wide-spread and easy testing than current practice and is also applicable to detection of multiple moieties for environmental and chemical detection applications. The proposed pilot testing of an interdisciplinary senior design project can lead to a sustainable model for engineering education. Proposed hands-on sensor units and associated experiments will lead to awareness of basic sensor principles and concepts among the participating school children. The proposed minority mentoring program can lead to recruitment into science and engineering programs.

这项研究的客观目标是开发一种传感器，用于检测多种生物标志物，并在早期检测和监测卵巢癌中进行了特定的应用。 该方法基于表面声波（SAW）换能器，该换能器集成了两个关键功能，即去除干涉蛋白，并在单个设备上传感多个生物标志物。技术优先项目是第一个提出并证明使用锯从传感器表面中去除非特异性结合（NSB）蛋白的项目。 NSB是使用任何传感原理，光学，电化学或质量变化的传感器响应的混杂因素。 提出的传感器是将此删除函数与任何设备的传感函数集成到的传感器。 这是一次一次尝试一次差异蛋白质的尝试，一次尝试一次性地去除单个设备表面上的多个部分的检测和定量。 许多疾病（例如卵巢癌）的特征是体液中精选蛋白水平升高。因此，该传感器将在医学诊断中具有广泛的适用性。 与当前的实践相比，这项工作更广泛的影响将允许进行更广泛和易于测试，并且还适用于检测多个部分的环境和化学检测应用。 跨学科高级设计项目的拟议试点测试可以导致工程教育的可持续模型。 拟议的动手传感器单元和相关的实验将导致对参与学童的基本传感器原理和概念的认识。 拟议的少数民族指导计划可以导致招募科学和工程计划。