Interfacial and Bulk Flow Processing of Biological Substrates

生物基质的界面和整体流处理

• 批准号: 0754060
• 负责人: Gerald Fuller
• 金额: $ 31.5万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-15 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0754060&HistoricalAwards=false
• 关键词: Interfacial; Bulk; Flow; Processing; Biological; Interfacial; Bulk; Flow; Processing; Biological

CBET-0754060FullerIntellectual Merit:Collagen and silk fibroin are fibrous proteins that have attracted interest as biomaterials for use as scaffold and cell culture substrates. This research effort is directed at interfacial and bulk flow processing methods that are able to control the orientation and morphology of these materials. It is demonstrated that this control of microstructure has important consequences on the behavior of human cells that are grown on the surfaces of these proteins. Interfacial flow processing methods will be investigated as a means of manipulating the orientation of collagen fibrils. In the case of silk fibroin, these techniques will be used to systematically vary the degree of crystallization and orientation of the crystalline morphology of this protein. Confinement of these proteins to an interface strongly facilitates intermolecular interactions and self assembly, and offers important advantages for flow-induced manipulation of microstructure. The second method that will be used to manipulate microstructure is the application of hydrodynamic forces to these materials from the liquid crystalline state. In the case of collagen, cholesteric phases can be achieved and flow processes are able to create remarkable structures through flow banding. These bands also appear to control cell growth. The experimental methods that will be applied to these systems include interfacial rheology to measure the surface moduli of these layers, dichroism measurements to determine flow-induced orientational order, the measurement of interfacial isotherms, and surface x-ray diffraction and reflectivity. The proposal includes preliminary data acquired using these techniques that reveal our ability to effectively create important, new morphologies with these fibrous proteins.The project has an REU and RET aspect.Broader Impacts Resulting from the Proposed Activity:Although this research effort is not a program in tissue engineering, its results are expected to be very relevant to this broad area. In particular, it is known that the morphology of substrate microstructure has a strong influence on the subsequent shape, size, and proliferation of cells that grow on them. Two graduate students in chemical engineering will be trained in subjects that include interfacial thermodynamics, interfacial fluid mechanics, rheology (bulk and interfacial), the culturing of cells, and an array of characterization techniques (interfacial rheology, dichroism and Brewster angle microscopy, and x-ray scattering). Outreach activities in the Fuller laboratory have provided internships to undergraduate and high school researchers.

CBET-0754060FullerIntlectual Furect：胶原蛋白和丝纤维蛋白是纤维蛋白，吸引了作为脚手架和细胞培养底物的生物材料的兴趣。这项研究工作针对界面和大量流动处理方法，能够控制这些材料的方向和形态。证明这种微观结构的控制对在这些蛋白质表面上生长的人类细胞的行为产生了重要影响。界面流动方法将被研究作为操纵胶原蛋白原纤维方向的一种手段。在丝绸纤维蛋白的情况下，这些技术将用于系统地改变该蛋白质的结晶形态的结晶程度和方向。将这些蛋白限制在界面上，强烈促进了分子间的相互作用和自组装，并为流动诱导的微观结构操纵提供了重要的优势。用于操纵微观结构的第二种方法是将流体动力应用于液晶状态的这些材料。在胶原蛋白的情况下，可以实现胆固醇相，流动过程能够通过流带创建出色的结构。这些频带似乎也控制了细胞的生长。将应用于这些系统的实验方法包括界面流变性，以测量这些层的表面模量，二色性测量，以确定流动诱导的定向阶，界面等温线的测量以及表面X射线衍射和反射率。该提案包括使用这些技术获得的初步数据，这些数据揭示了我们使用这些纤维蛋白有效创造重要的新形态的能力。该项目具有REU和RET方面。Boader构成了拟议活动的影响：尽管这项研究工作与组织工程中的计划无关，但其结果预计与这一广泛领域非常相关。特别是，众所周知，底物微观结构的形态对生长在其上的细胞的随后形状，大小和增殖具有很大的影响。两名化学工程研究生将​​接受包括界面热力学，界面流体力学，流变学（大量和界面），细胞培养以及一系列特征技术（互流性技术，二色症，二色症和布鲁斯特角度显微镜和X射线散射）的受试者。富勒实验室的外展活动为本科和高中研究人员提供了实习。