Effects of negative energy balance on muscle mass regulation

负能量平衡对肌肉质量调节的影响

• 批准号: 184034212
• 负责人: Professor Dr. Joachim Spranger
• 金额: --
• 依托单位: Medizinische Klinik für Endokrinologie und Stoffwechselmedizin (Diabetes und Ernährungsmedizin)
• 依托单位国家: 德国
• 项目类别: Clinical Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/184034212?language=en
• 关键词: Effects; negative; energy; balance; muscle

Skeletal muscle is pivotal to the body’s metabolism. Losses of skeletal muscle mass have been proposed to predict mortality. Oddly, the regulation of muscle mass itself has accrued relatively little attention. A network of neuro-endocrine factors tightly regulates energy balance and fat mass. An analogous neuro-endocrine network likely contributes to the regulation of muscle mass. Intentional weight loss (dieting) invariably leads to muscle mass losses. We hypothesize that intentional weight loss modifies the responsible regulatory neuro-endocrine network. We will analyze changes in muscle mass and function during and after intentional weight loss. We will dissociate effects of negative energy balance and those actions, which result from a modification of body composition. To address these topics, we will perform a randomized controlled weight reduction trial in 60 post-menopausal women. We will study the subjects before, during, and after weight reduction (with stable weight and after weight regain). Phenotyping will include a detailed analysis of potential endocrine parameters involved in the regulation of muscle mass. Detailed metabolic in-vivo characterization including microdialysis, energy balance, clamps, MRI imaging and ex-vivo analysis of human myotubes from each participant using targeted and unbiased technologies, such as expression profiling will be included. These analyses will improve our understanding of the endocrine regulation of muscle mass and may offer novel endocrine or metabolic targets to avoid the well known side effect of muscle mass reduction during weight loss interventions. We raise the provocative notion that short-term dieting (being clinical reality) could be detrimental, since weight regain is not necessarily accompanied by regain of muscle mass. Furthermore, we will examine the mechanisms responsible for any counterproductive effects.

骨骼肌对于身体的新陈代谢至关重要。骨骼肌质量的损失被认为可以预测死亡率，但奇怪的是，肌肉质量本身的调节却相对较少受到关注。类似的神经内分泌网络可能有助于肌肉质量的调节，有意减肥（节食）必然会导致肌肉质量损失。我们发现，有意减肥会改变负责任的调节作用。我们将分析有意减肥期间和之后肌肉质量和功能的变化，我们将分离负能量平衡的影响以及身体成分改变所产生的影响。一项针对 60 名绝经后女性的随机对照减重试验，我们将研究减重之前、期间和之后的受试者（体重稳定和体重恢复后），表型分析将包括对参与调节的潜在内分泌参数的详细分析。肌肉的详细的体内代谢特征，包括微透析、能量平衡、钳夹、MRI 成像和使用参与者定向和公正的技术（例如表达谱）进行的体外分析，这些分析将提高我们对质量的理解。肌肉质量的内分泌调节，并可能提供新的内分泌或代谢目标，以避免减肥干预期间众所周知的肌肉质量减少的副作用。现实）可能会令人痛苦，因为体重恢复不一定伴随着肌肉质量的恢复。此外，我们将研究导致任何反作用的机制。