MRI: Acquisition of a Stimulated Emission Depletion (STED) Microscope for Nanoscopic Resolution of Biological Samples

MRI：获取受激发射损耗 (STED) 显微镜以实现生物样品的纳米级分辨率

• 批准号: 0722519
• 负责人: Shimon Weiss
• 金额: $ 110万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0722519&HistoricalAwards=false
• 关键词: MRI; Acquisition; Stimulated; Emission; Depletion

The Chemistry Department at the University of California-Los Angeles will acquire a stimulated emission depletion confocal laser scanning microscope (STED-CLSM) with this award from the Major Research Instrumentation (MRI) program. The requested microscope displays resolution down to 28 nm in the focal plane, a 10x improvement over conventional light microscopes. The instrument will be used to develop multi-color inorganic, stable, quantum rods as novel STED probes, decipher the structure of chromatin and its packaging into chromosomes in the cell, study cell signaling, viral and bacterial infection pathways, neural plasticity and many other important biological questions.STED microscopy provides an alternative to electron microscopy because it capitalizes on the well-established advantages of fluorescence microscopy (sensitivity, molecular specificity, genetically encoded probes, live cells, ease of operation). The STED concept relies on a purely physical phenomenon, stimulated emission, coupled with smart optics, to sharpen the confocal excitation spot, resulting in much more detailed, nanometer resolved images. Bridging the gap between electron and diffraction-limited light microscopy, a STED nanoscope should be a powerful tool for unraveling the relationship between structure and function in cell biology. Indeed, many outstanding problems lay in the nanometer scale, such as the organization of chromosomes, the assembly of large protein complexes and viral structures, organelle structures, as well as applications to non-biological nano-scale devices.

加州大学洛杉矶分校的化学系将通过主要研究仪器（MRI）计划获得刺激的排放耗尽共聚焦激光扫描显微镜（STED-CLSM）。 所请求的显微镜在焦平面中显示到28 nm的分辨率，比传统的光显微镜提高了10倍。 该仪器将用于开发多色无机，稳定的量子杆作为新型Sted探针，将染色质的结构及其包装解码为细胞中的染色体，研究细胞信号传导，病毒和细菌感染途径，神经形态以及许多其他重要的生物学问题。STED显微镜提供了电子显微镜的替代方法，因为它大写了荧光显微镜（灵敏度，分子特异性，遗传编码的探针，活细胞，易于操作）的良好优势（敏感性，分子特异性，易于操作）。 STED概念依赖于纯粹的物理现象，刺激的发射以及智能光学元件来锐化共聚焦激发点，从而产生了更详细的纳米分析图像。 弥合电子和衍射限制的光学显微镜之间的间隙，steD纳米镜应该是揭示细胞生物学中结构与功能之间关系的强大工具。 实际上，许多未出现的问题在于纳米量表，例如染色体的组织，大蛋白质复合物和病毒结构的组装，细胞器结构以及对非生物纳米级设备的应用。