Minority Postdoctoral Research Fellowship for FY2006

2006财年少数族裔博士后研究奖学金

• 批准号: 0610251
• 负责人: Jonathan Awaya
• 金额: --
• 依托单位: Awaya Jonathan D
• 依托单位国家: 美国
• 项目类别: Fellowship
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0610251&HistoricalAwards=false
• 关键词: Minority; Postdoctoral; Research; Fellowship; FY2006

This project is awarded under the Minority Postdoctoral Research Fellowships and Supporting Activities Program for 2006. IRON ACQUISITION BY PSEUDOMONAS MENDOCINA STRAIN YMPIron is the most abundant element on earth and perhaps not surprising that most forms of life have evolved an obligate dependence on iron. However, under aerobic conditions the majority of iron exists as insoluble ferric mineral forms. In response to such environmental pressures, prokaryotes have evolved strategies such as the use of siderophores for sequestering iron from a limited soluble supply. Siderophores and siderophore-producing microbes have been shown to have significant effects on rates of mineral dissolution. Mineral stability at DOE Nevada test sites, particularly those proposed as storage sites for mineralized radionuclide waste, is clearly critical. The fundamental interest in microbial contributions to iron cycling is therefore compounded by concern over possible unwanted repercussions of microbe-mineral interactions. Isolated from the DOE site, Pseudomonas mendocina strain ymp has been studied for siderophore production and mineral dissolution. However, very little is known about the genetic content of any strain of P. mendocina. The goals of this project are to identify, classify, and organize genes involved in iron uptake by both siderophore-mediated pathways, and by unanticipated pathways. Finally in the process of meeting these aims, a number of genetic and molecular biological tools that will make P. mendocina more amenable for future biogeochemical investigations will be developed. The research will be conducted in the laboratory of Dr. Jennifer DuBois at Univeristy of Notre Dame. Dr. Awaya aims through the NSF Minority Fellowship, to strengthen his skills as a molecular microbiologist and to provide an alternative approach to understand microbe-mineral interactions. The main objective of this postdoctoral training is to construct a solid foundation for him to become a leading environmental scientist in molecular microbiology in the future. He will attend several scientific meetings to present his research findings, make personal contacts for future collaborations and receive feedback to strengthen his abilities as a researcher in the scientific community. One of his plans is to be able to establish his own laboratory and teach at a univeristy.

该项目是根据2006年的少数族裔博士后研究奖学金和支持活动计划授予的。假单胞菌Mendocina Strain Ympiron获得的铁收购是地球上最丰富的元素，并且可能不足为奇的是，大多数形式的生活已经发展出对铁的义务依赖性。 但是，在有氧条件下，大多数铁作为不溶性铁矿物形式存在。为了应对这种环境压力，原核生物已经发展出策略，例如使用铁载体来隔离有限的可溶供应铁。 辅助载体和产生铁载体的微生物已被证明对矿物质溶解速率具有重大影响。 内华达州能源部测试地点的矿物质稳定性，尤其是那些作为矿物质放射性核素废物存储地点的矿物质稳定性显然至关重要。因此，对微生物循环的微生物贡献的基本兴趣是对微生物矿物质相互作用可能不良影响的担忧。已从DOE部位分离出，已研究了Mendocina菌株YMP的假单胞菌菌株YMP，以用于铁载体的产生和矿物质溶解。 然而，对蒙多西纳菌菌株的遗传含量知之甚少。该项目的目标是通过两种铁载体介导的途径以及意外途径来识别，分类和组织参与铁吸收的基因。 最终，在满足这些目标的过程中，将开发许多遗传和分子生​​物学工具，这些工具将使孟多迪科纳更适合未来的生物地球化学研究。 这项研究将在巴黎圣母院大学的詹妮弗·杜波依斯博士实验室进行。 Awaya博士的目标是通过NSF少数群体奖学金，以增强他作为分子微生物学家的技能，并提供一种理解微生物相互作用的替代方法。 这种博士后培训的主要目的是为他在将来成为分子微生物学领域的主要环境科学家奠定坚实的基础。 他将参加几次科学会议，以介绍他的研究结果，进行个人联系以进行将来的合作，并获得反馈，以增强他作为科学界研究人员的能力。他的计划之一是能够建立自己的实验室并在大学上任教。