NIRT: Active Nanostructures for Nucleic Directed synthesis of Organic Functional Polymers

NIRT：用于有机功能聚合物核定向合成的活性纳米结构

• 批准号: 0608889
• 负责人: Nadrian Seeman
• 金额: $ 150万
• 依托单位: New York University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-10-01 至 2011-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0608889&HistoricalAwards=false
• 关键词: NIRT; Active; Nanostructures; Nucleic; Directed

PI: Nadrian C. Seeman Institution: New York UniversityProposal Number: 0608889Title: NIRT: Active Nanostructures for Nucleic Directed Synthesis of Organic Functional PolymersThis proposal was received in response to Nanoscale Science and Engineering initiative, NSF 05-610, category NIRT. Summary: The goal of this project is to build on the DNA nanotechnology developed by the Seeman laboratory over the last four years to chemically manufacture a DNA nanomachine capable of synthesizing polymers with desired optical, electrical, and stereochemical properties that are difficult or impossible to achieve with normal polymer synthesis techniques. The aim is to develop and prototype the strategy to construct such polymers with a precision approaching that by which the ribosome constructs proteins. This project would bring to bear underlying tools that have been developed recently by Seeman (NYU), Goddard (Caltech), Winfree (Caltech), and Canary (NYU) under previous NIRT and SGER projects. They plan to use these tools to produce and demonstrate the artificial ribosome device. To accomplish this they plan to:(1) Develop a translation device to convert a given sequence of monomers to polymers with precise length and sequence control (modifying the rotary device previously developed by Seeman)(2) Use transcriptional circuitry developed by Winfree to control the nature and sequence of the polymer synthesized and to provide polymers that reflect the history of the system.(3) Use multiscale first principles-based simulation tools developed by Goddard and Deng to design and optimize the polymer monomers and connections to achieve supramolecular active organic polymer structures with desired electronic or optical properties(4) Use novel organic synthesis techniques developed by Canary to build DNA-polymer subunit conjugates to add polymer subunits to nucleic acid backbones. This chemistry-prototyping system already responds to a continuous message.They plan to first introduce a translocation step in the nanodevice so that inputting a new message to the device will result in a new polymer product. The advantage of translocation (in contrast to the rotary device already developed by Seeman), is that longer products can be built without making larger devices. Ultimately they expect to generate the message via the transcriptional circuitry developed in the Winfree laboratory, thereby building polymers as a consequence of logical circuitry and in response to cues from the surrounding medium.Intellectual Merit: This project attempts to develop a prototype DNA based machine capable of synthesizing organic polymers with the same flexibility and specificity displayed by the ribosome in synthesizing proteins. This is a grand challenge which would stimulate a number of research efforts to enable this for broader ranges of organic compounds. Nothing in the technology limits it to organic polymers. The project builds on many years of development in the Seeman, Goddard, Winfree, and Canary labs all to be brought to bear on this problem. The confluence of these disparate efforts would likely stimulate each of the research areas involved. To provide a useful target for this technology, the PIs plan to develop novel one and two dimensional nonlinear optical (NLO) polymers with very large hyperpolarizabilities (susceptibilities). Even more ambitious is the proposal to build a new type of nanostructure architecture based on assembling a 2D array of functional polymers (rather than the nanotubes or nanowires of other recent approaches). They suggest that these can be used to put logic units at the nodes rather than simple switches. This should stimulate many new ideas and proposals.Broader Impacts: The NYU and Caltech PIs have aggressively pursued the active incorporation of underrepresented groups within their research operations and they have participated in special outreach programs aimed at both high school and K6 students with an emphasis on underrepresented groups. They are strongly committed to continuing and escalating these efforts under the NIRT program. For example, the Goddard group has played a vital role in Quality Education for Minorities (QEM) and Minorities in Mathematics, Science, and Engineering (MSE) programs at Caltech. NYU has involved many high school students in nanotechnology research and Seeman works closely with scientifically-oriented arts and entertainment groups.Research and Education Theme: Nanoscale Devices and System Architectures

PI：Nadrian C. Seeman机构：纽约大学港口编号：0608889TITLE：NIRT：用于核定的有机功能聚合物核定纳米结构，这是对纳米级科学和工程倡议的响应，NSF 05-610，NSF 05-610，类别。 摘要：该项目的目的是建立在过去四年中，Seeman实验室开发的DNA纳米技术建立在化学生产的DNA纳米机械上，该DNA纳米机械能够通过正常聚合物合成技术来合成所需的光学，电和立体化学性能，这些纳米机构具有所需的光学，电和立体化学性能。 目的是制定和原型构建此类聚合物的策略，以核糖体构建蛋白质的精确度接近。 该项目将带来由Seeman（NYU），Goddard（Caltech），Winfree（Caltech）和Canary（NYU）在以前的NIRT和SGER项目下开发的基础工具。 他们计划使用这些工具生产和演示人造核糖体设备。 为了实现这一目标，他们计划：（1）开发一种翻译装置，将给定的单体序列转换为具有精确的长度和序列控制的聚合物（修改先前由Seeman开发的旋转设备）（2）使用由winfree开发的转录电路，以控制型和构建的聚合物的跨性别型和跨度的原理，以反映越多的组合的原理。设计和优化聚合物单体和连接，以实现具有所需电子或光学性能的超分子活性有机聚合物结构（4）使用Canary开发的新型有机合成技术来构建DNA - 聚合物亚基甲轭物来将聚合物亚基添加到核酸骨架中。该化学跨性型系统已经响应连续的消息。他们计划在纳米电视中首先引入易位步骤，以便将新消息输入到设备上将导致新的聚合物产品。 易位的优点（与Seeman已经开发的旋转设备相比），即可以在不制造更大设备的情况下构建更长的产品。 最终，他们期望通过在无条件实验室中开发的转录电路来产生信息，从而构建聚合物是由于逻辑电路的结果以及对周围介质的提示的响应。启动性优点：该项目试图开发基于原型DNA的机器，能够通过同一柔韧性和特异性蛋白质来开发一种能够合成的有机聚合物，并通过果实构成了核心。 这是一个巨大的挑战，它将刺激许多研究工作，以使其能够为更广泛的有机化合物范围启用。 技术中的任何内容均不将其限制在有机聚合物中。 该项目建立在Seeman，Goddard，Winfree和Canary Labs的多年发展，所有这些都将在这个问题上遇到。 这些不同的努力的汇合可能会刺激每个研究领域。 为了为这项技术提供有用的目标，PIS计划开发具有非常大的超极化能力（敏感性）的新型一维非线性光学（NLO）聚合物。 更雄心勃勃的是建立一种基于组装2D功能聚合物（而不是其他最近方法的纳米管或纳米线）的新型纳米结构架构的建议。 他们建议这些可用于将逻辑单元放在节点，而不是简单的开关。 这应该刺激许多新的想法和建议。BROADER的影响：纽约大学和加州理工学院PIS积极地追求了他们的研究行动中代表性不足的群体的积极纳入，他们参加了针对高中和K6学生的特殊外展计划，并重点是代表性不足的群体。他们强烈致力于在NIRT计划下继续并升级这些努力。 例如，戈达德集团在加州理工学院（Caltech）的数学，科学和工程（MSE）计划中在少数民族（QEM）和少数民族的质量教育中发挥了至关重要的作用。 纽约大学（NYU