IDBR: Development of an Electrostatic Ion Trap Tandem Mass Spectrometer

IDBR：静电离子阱串联质谱仪的开发

• 批准号: 0551958
• 负责人: Arthur Suits
• 金额: --
• 依托单位: Wayne State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0551958&HistoricalAwards=false
• 关键词: IDBR; Development; Electrostatic; Ion; Trap

This award supports a new approach to tandem mass spectrometry based upon the emerging technology of electrostatic ion traps and use of electron capture (EC) and vacuum ultraviolet (VUV) radiation for dissociation. This is expected to afford key advantages in many important biological applications over existing tandem mass spectrometers. Electron capture dissociation, which has been shown to preserve many labile post-translational protein modifications, has recently emerged as an alternative to commonly used collisional fragmentation methods. However, its use is currently restricted to costly Fourier transform ion cyclotron resonance mass spectrometers owing to incompatibility with conventional RF ion traps. With the advent of simple electrostatic ion traps recently reported, there is a new opportunity for using less harsh fragmentation such as that provided by EC or VUV, as a basis for tandem mass spectrometry. The result will be a device that is simple and relatively inexpensive to construct and at the same time amenable to incorporation of both electron capture and laser generated ultraviolet radiation to fragment large biomolecules. The inherently interdisciplinary nature of this work will promote training of scientists ideally equipped to address the challenges facing the chemical and biological sciences in the coming decades. For the post-doc and graduate students directly involved, it will simultaneously give them deep expertise in the development of state-of-the art mass spectrometry hardware, steep them in the practical issues in ion chemistry and fragmentation mechanisms, and expose them to the essentials of protein purification, sequencing and sample preparation. For the undergraduates, it will provide invaluable hands-on experience in design, construction, and demonstration of a new approach to tandem mass spectrometry. Wayne State University, with the largest African-American population of any nonhistorically Black university, has a number of outreach programs to enlist underrepresented minorities in science education and training. The present proposal provides an excellent opportunity to take advantage of these programs at the undergraduate and graduate levels as well as in outreach to the Detroit community.

该奖项支持基于静电离子阱新兴技术以及使用电子捕获 (EC) 和真空紫外 (VUV) 辐射进行解离的串联质谱新方法。与现有的串联质谱仪相比，这有望在许多重要的生物应用中提供关键优势。 电子捕获解离已被证明可以保留许多不稳定的翻译后蛋白质修饰，最近已成为常用碰撞断裂方法的替代方法。然而，由于与传统的射频离子阱不兼容，目前其使用仅限于昂贵的傅里叶变换离子回旋共振质谱仪。随着最近报道的简单静电离子阱的出现，出现了使用不太苛刻的碎裂（例如 EC 或 VUV 提供的碎裂）作为串联质谱分析的基础的新机会。其结果将是一种结构简单且相对便宜的装置，同时可以结合电子捕获和激光产生的紫外线辐射来破碎大的生物分子。这项工作固有的跨学科性质将促进对科学家的培训，使他们能够应对未来几十年化学和生物科学面临的挑战。对于直接参与的博士后和研究生，它将同时为他们提供最先进的质谱硬件开发方面的深厚专业知识，让他们深入了解离子化学和碎片机制的实际问题，并使他们接触到蛋白质纯化、测序和样品制备的要点。对于本科生来说，它将在设计、构建和演示串联质谱新方法方面提供宝贵的实践经验。韦恩州立大学是所有非历史黑人大学中非裔美国人人口最多的大学，该大学制定了许多外展计划，以吸引代表性不足的少数族裔参与科学教育和培训。目前的提案提供了一个绝佳的机会，可以在本科生和研究生阶段以及向底特律社区推广这些项目。