NER: Investigation and Characterization of Adsorption of Self Assembling Amyloid Beta Protein Aggregates on Gold Nanoparticles Embedded in a Sol-Gel Thin Film

NER：溶胶-凝胶薄膜中嵌入的金纳米颗粒上自组装淀粉样β蛋白聚集体吸附的研究和表征

• 批准号: 0508240
• 负责人: Kazushige Yokoyama
• 金额: $ 7.5万
• 依托单位: SUNY College at Geneseo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0508240&HistoricalAwards=false
• 关键词: NER; Investigation; Characterization; Adsorption; Self

We propose to investigate the application of gold nanoparticles as an effective marker for nuclei formation of amyloid b-peptides (Ab), which is a hallmark of Alzheimer's disease. The goal of this project is to establish a diagnostic method to relate the size of nanoparticles with the size of the assembled Ab arising from the aggregation process. The objectives are 1) to compile a spectroscopic characterization of gold nanoparticles when they coexist with Ab, 2) to find an indication of adsorption or self-assembly of Ab aggregates in a silica sol-gel matrix in thin film form. As aggregation of the protein takes place, light scattering will be strongly enhanced. Thus, we will adopt a surface reflectance diffuse spectroscopy technique, which is well suited for measuring the absorption spectroscopic information of the scattering surface area. The spectroscopic information will be correlated with the absorption information of gold nanoparticle complexes alone, and the spectral shift used for an estimation of the aggregation size. As a broader impact of the proposed study, the present study may result in a correlation scheme for protein conformation adsorbed on gold nanoparticles, which could be used to establish a standard diagnostic methodology for a protein aggregation. Through this research project, three undergraduate students will have an opportunity to be engaged in an application of nanopartcile science and they have an opportunity to use highly specialized instruments, such as an Atomic Force Microscope at the Center for Advanced Technology in Nanomaterials and Nanoelectronics, State University of New York at Albany.

我们建议研究金纳米粒子作为淀粉样 B 肽 (Ab) 核形成的有效标记物的应用，Ab 是阿尔茨海默病的标志。 该项目的目标是建立一种诊断方法，将纳米颗粒的尺寸与聚集过程中产生的组装抗体的尺寸联系起来。 目标是 1) 编制金纳米粒子与 Ab 共存时的光谱表征，2) 找到薄膜形式的二氧化硅溶胶-凝胶基质中 Ab 聚集体吸附或自组装的指示。 当蛋白质发生聚集时，光散射将强烈增强。 因此，我们将采用表面反射漫反射光谱技术，该技术非常适合测量散射表面区域的吸收光谱信息。 光谱信息将与单独的金纳米颗粒复合物的吸收信息以及用于估计聚集体尺寸的光谱位移相关。 作为拟议研究的更广泛影响，本研究可能会产生金纳米颗粒上吸附的蛋白质构象的相关方案，该方案可用于建立蛋白质聚集的标准诊断方法。 通过这个研究项目，三名本科生将有机会从事纳米粒子科学的应用，并有机会使用高度专业化的仪器，例如国家纳米材料和纳米电子学先进技术中心的原子力显微镜纽约大学奥尔巴尼分校。