Effects of nanomolar heavy metal concentrations on plants - comparison of biochemical&biophysical mechanisms of deficiency and sublethal toxicity under environmentally relevant conditions

纳摩尔重金属浓度对植物的影响——生化比较

基本信息

批准号：
169833033
负责人：
Professor Dr. Hendrik Küpper
金额：
--
依托单位：
Deutsches Elektronen-Synchrotron (DESY)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2010
资助国家：
德国
起止时间：
2009-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/169833033?language=en
关键词：
Effects nanomolar heavy metal concentrations

项目摘要

In this project we are investigating mechanisms of heavy metal toxicity and deficiency. We started this in the water plant Ceratophyllum demersum as a model for shoots, and now also transfer the knowledge obtained from this model to crop plants. While many potential mechanisms of metal toxicity induced damage have been proposed, most of them were investigated only under rather artificial laboratory conditions. Therefore, we are evaluating the relevance of different proposed mechanisms of metal-induced inhibition under environmentally relevant conditions and are comparing these results with those obtained under conditions that were used in identifying the putative toxicity mechanisms. We have chosen copper, chromium and cadmium as model heavy metals, plus arsenic as a toxic metalloid, because they most frequently reach toxic concentrations in contaminated environments. We apply environmentally relevant conditions in terms of concentrations, biomass/water volume ratios, light and temperature cycles and incubation times. In the past 39 months of the project, the focus was at the beginning on the analysis of the toxicity of lake Ammelshain samples and the determination of reasons for their toxicity by systematic re-constitution of field conditions in the laboratory. Afterwards we systematically investigated mechanisms of deficiency of copper and chromium, as well as toxicity of arsenic, cadmium and copper. The deficiency & toxicity analysis was started with in vivo measurements of photosynthesis biophysics, formation of reactive oxygen species and oxygen exchange, combined with pigment, starch and metal analyses, and is currently being continued by analysis of cellular and subcellular metal distribution and speciation, as well as metalloproteomics. In the coming years, we want to transfer the knowledge obtained with the model plant to crop species (mainly soybean and barley), with special emphasis on cellular and subcellular metal distribution (via µXRF) and speciation (via µXANES), as well as metalloproteomics and metabolomics. Thus we want to investigate further how in plants metal deficiency and onset of metal toxicity lead to a change in metal distribution between different tissues, different cellular compartments and different metal-binding proteins, rather than a general concentration change in many tissues. Further, we want to clone the genes of novel metal-binding proteins to further characterise them and reveal their function by modifying their expression in the crop species and Arabidopsis thaliana. This particularly applies the proteins that bind Cr under optimal and Cr-deficient conditions, where we already have a preliminary identification of one of these proteins.

在这个项目中，我们正在研究重金属毒性和缺乏的机制，我们开始在水生植物金鱼藻中作为芽模型，现在也将从该模型中获得的知识转移到作物植物中，同时许多潜在的金属毒性机制。已提出诱导损伤，其中大多数仅在相当人工的实验室条件下进行研究，因此，我们正在评估环境相关条件下金属诱导抑制的不同提议机制的相关性，并将这些结果与在环境相关条件下获得的结果进行比较。用于确定假定的毒性机制。我们选择铜、铬和镉作为模型重金属，并选择砷作为有毒类金属，因为它们在受污染的环境中最常达到有毒浓度。我们在浓度、生物量/水体积比、光照和温度方面应用环境相关条件。在过去 39 个月的项目中，重点是分析阿梅尔斯海因湖样品的毒性并通过系统重建现场确定其毒性原因。随后，我们系统地研究了铜和铬的缺乏机制，以及砷、镉和铜的毒性。通过体内测量光合作用生物物理学、活性氧和氧的形成来开始缺乏和毒性分析。交换，与色素、淀粉和金属分析相结合，目前正在继续分析细胞和亚细胞金属分布和形态以及金属蛋白质组学。在未来几年，我们希望转移通过分析获得的知识。模型植物到作物物种（主要是大豆和大麦），特别强调细胞和亚细胞金属分布（通过 µXRF）和物种形成（通过 µXANES），以及金属蛋白质组学和代谢组学，因此我们希望进一步研究金属植物中的情况。金属毒性的缺乏和发生会导致不同组织、不同细胞区室和不同金属结合蛋白之间的金属分布发生变化，而不是许多组织中的总体浓度变化此外，我们希望克隆这些基因。新型金属结合蛋白的研究，通过改变它们在作物物种和拟南芥中的表达来进一步表征它们并揭示它们的功能，这特别适用于在最佳和缺铬条件下结合 Cr 的蛋白质，我们已经初步鉴定了这些蛋白质。这些蛋白质之一。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Different strategies of cadmium detoxification in the submerged macrophyte Ceratophyllum demersum L.

沉水植物金鱼藻的不同镉解毒策略。

DOI：
10.1039/c3mt00088e
发表时间：
2013-09-26
期刊：
Metallomics : integrated biometal science
影响因子：
0
作者：
E. Andresen;J. Mattusch;G. Wellenreuther;G. Thomas;Uriel Arroyo Abad;H. Küpper
通讯作者：
H. Küpper

Speciation and Distribution of Arsenic in the Nonhyperaccumulator Macrophyte Ceratophyllum demersum1[C][W]

非超富集植物金鱼藻1中砷的形态与分布[C][W]