Electronic Detection of BioMolecules

生物分子的电子检测

• 批准号: 0415130
• 负责人: George Gruner
• 金额: $ 39万
• 依托单位: University of California-Los Angeles
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-15 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0415130&HistoricalAwards=false
• 关键词: Electronic; Detection; BioMolecules

This project focuses on the investigation of conformational dynamics of biomolecules using nanoscale electronic structures where the sensing element is a carbon nanotube or a nanotube network. Such sensors are small and fast (the active area is compatible with individual proteins or DNA) and are extremely sensitive since all the current flows at the surface of the nanotube conducting channel, and thus passes through the detection point. The objective of the research is to use these nanoscale electronic devices as bio-sensors and also explore their potential in real-time detection of conformational changes of biomolecules in a buffer environment. Three areas will be explored. First, duplex formation as well as the melting of the DNA duplex will be examined using oligomers of different lengths and sequences. Second, interactions between proteins will be studied using the biotin-streptavidin, ligand-receptor model system. The feasibility of detecting conformational changes will be explored using bacteriorhodopsin as a model system. Third, direct detection of protein binding to nanotubes, and the subsequent conformational changes, will be monitored in real time using devices where individual nanotubes span the source and drain of the electronic device. Experiments on electronic detection will be conducted hand-in-hand with spectroscopic measurements and with direct imaging of the devices. In the broader sense, electronic detection schemes may offer alternatives of optical detection in diverse areas of biotechnology, including gene chips and protein chips, and also medical diagnostics. The electronic detection schemes to be explored may complement other single molecule detection techniques, including optical methods, ESR and NMR techniques.

该项目的重点是使用纳米级电子结构对生物分子的构象动力学进行研究，其中传感元件是碳纳米管或纳米管网络。这样的传感器很小且快速（活动区域与单个蛋白质或DNA兼容），并且非常敏感，因为所有电流流动在纳米管传导通道的表面上，因此通过检测点。该研究的目的是将这些纳米级电子设备用作生物传感器，并探索它们在缓冲液环境中生物分子构象变化的实时检测潜力。将探索三个区域。首先，将使用不同长度和序列的低聚物检查双链体的形成以及DNA双链体的熔化。其次，将使用生物素 - 链霉替蛋白，配体受体模型系统研究蛋白质之间的相互作用。检测构象变化的可行性将使用细菌紫红素作为模型系统探索。第三，将直接检测蛋白质与纳米管结合，然后使用单个纳米管跨越电子设备的源和排水的设备，实时监测随后的构象变化。电子检测的实验将与光谱测量并直接对设备进行直接成像进行。从更广泛的意义上讲，电子检测方案可以在生物技术的不同领域（包括基因芯片和蛋白质芯片以及医学诊断）提供光学检测的替代方法。要探索的电子检测方案可能会补充其他单分子检测技术，包括光学方法，ESR和NMR技术。