Collaborative Research: Developmental, Molecular and Evolutionary Analysis of Chaetognaths: A Deuterostome-Like Ecdysozoan

合作研究：毛颌动物的发育、分子和进化分析：类似后口动物的蜕皮动物

• 批准号: 0333843
• 负责人: Kenneth Halanych
• 金额: --
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0333843&HistoricalAwards=false
• 关键词: Collaborative; Research; Developmental; Molecular; Evolutionary

0236479HalanychThe chaetognaths, or "arrow worms", are one of the most abundant marine invertebrates found in all the world's seas. The phylogenetic position of this ubiquitous group of animals is currently unknown. Until recently, chaetognaths were considered to be basal deuterostomes based on developmental features such as their radial cleavage program, classic enterocoeley, posterior position of the blastopore, and tripartite adult body plan. New molecular evidence, including Hox gene data presented in this proposal, has suggested that chaetognaths are not deuterostomes but basal protostomes closely allied with ecdysozoans such as arthropods and nematodes. In fact, chaetognaths are now thought to be one of the most pivotal and understudied groups of metazoan animals for gaining insight into the evolution of protostome body plans. Only a handful of cellular and molecular investigations of chaetognath development have ever been made, however. Drs. Martindale and Halanych's proposal describes a comprehensive and multidisciplinary set of approaches designed to characterize how chaetognath embryos "work". Using both modern developmental (intracellular cell lineage labeling and laser cell deletion experiments) and molecular (cloning, in situ hybridization) techniques, Martindale and Halanych will collect vital information on the normal development of these interesting animals, and be able to and examine the conservation of genetic regulatory mechanisms in the Metazoa. In addition, techniques of gene discovery (EST screening) will provide valuable new information on the ancestral mechanisms of body plan evolution. These data will not only be important to determine the phylogenetic position of chaetognaths, but to also provide molecular and developmental insight in to the evolution of the ecdysozoan clade, and the "evolveability" of developmental programs. Chaetognaths could be the most informative and tractable basal invertebrate system available that will allow an understanding of the evolution of the nematode and arthropod clades, the two most genetically heavily studied group of animals on earth.

0236479 Halanychthe Chaetognaths或“箭头蠕虫”是世界上所有海洋中发现的最丰富的海洋无脊椎动物之一。 目前，这种无处不在的动物群体的系统发育位置尚不清楚。 直到最近，基于发育特征，例如其径向裂解程序，经典肠球菌，胚孔波的后置位置和三方成人身体计划，才被认为是基础氘代表。 新的分子证据，包括本提案中介绍的HOX基因数据，它表明，Chaetognaths不是氘代表，而是基础原始原生质体与eCdysozoans（例如节肢动物和线虫）紧密相关。 实际上，现在认为Chaetognaths是最重要，最研究的后生动物群体之一，可以深入了解原始身体计划的演变。但是，仅进行了少数几个细胞和分子研究。 博士。 Martindale和Halanych的提议描述了一种旨在表征Chaetognath胚胎“工作”的全面和多学科的方法。使用现代发育（细胞内细胞谱系标记和激光细胞缺失实验）和分子（克隆，原位杂交）技术，Martindale和Halanych将收集有关这些有趣动物的正常发育的重要信息，并能够能够和检查元唑元中遗传调节机制的保护。 此外，基因发现技术（EST筛选）将提供有关身体计划进化的祖先机制的宝贵新信息。这些数据不仅对于确定Chaetognaths的系统发育位置非常重要，而且还为Ecdysozoan进化枝的演变提供了分子和发育见解，以及发展计划的“可探测性”。 Chaetognaths可能是可用的最有用，最有用的基础无脊椎动物系统，它将允许了解线虫和节肢动物进化枝的演变，这是地球上两个最广泛研究的动物。