CAREER: Solid-State NMR of Half-Integer Quadrupolar Vanadium Sites in Vanadium Haloperoxidases

职业生涯：钒卤过氧化物酶中半整数四极钒位点的固态核磁共振

• 批准号: 0237612
• 负责人: Tatyana Polenova
• 金额: $ 50.9万
• 依托单位: CUNY Hunter College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-03-01 至 2003-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0237612&HistoricalAwards=false
• 关键词: CAREER; Solid; State; NMR; Half

In this CAREER project funded by the Experimental Physical Chemistry Program of the Chemistry Division, Polenova will study vanadium-containing enzymes and associated model compounds using a variety of NMR techniques. The NMR experiments will be interpreted using density matrix simulations of spectra, and the electric quadrupole gradients for the vanadium will be interpreted using parameterized electronic structure calculations. These methods will be applied to haloperoxidases as well as to studies of vanadyl model compounds with known crystal structure.Metal ions serve a number of purposes in proteins, including catalytic, storage, and structural functions. Many of the mechanistic aspects behind these functions are not well understood. The application of solid-state NMR to metal sites in proteins is an important application. In this work Polenova is able to gather data about metal complexes with high sensitivity and high resolution. These data should have an impact on understanding biologically relevant systems as well as helping advance the state of knowledge at the interface between spectroscopy, bioinorganic chemistry, and metallobiochemistry. The results have the potential to help develop more environmentally friendly methods of catalysis. In her research program Polenova involves not only graduate and undergraduate students, but she also performs outreach to high school students. In addition, her education activities encourage underrepresented groups to pursue careers in science.

在这个由化学部实验物理化学项目资助的职业项目中，Polenova 将使用各种 NMR 技术研究含钒酶和相关模型化合物。 核磁共振实验将使用光谱的密度矩阵模拟进行解释，钒的电四极梯度将使用参数化电子结构计算进行解释。 这些方法将应用于卤过氧化物酶以及具有已知晶体结构的氧钒基模型化合物的研究。金属离子在蛋白质中具有多种用途，包括催化、储存和结构功能。 这些功能背后的许多机制尚不清楚。 固态核磁共振在蛋白质金属位点上的应用是一个重要的应用。 在这项工作中，Polenova 能够以高灵敏度和高分辨率收集有关金属配合物的数据。 这些数据应该对理解生物学相关系统产生影响，并有助于提高光谱学、生物无机化学和金属生物化学之间的知识水平。 研究结果有可能帮助开发更环保的催化方法。 Polenova 的研究项目不仅涉及研究生和本科生，还向高中生进行推广。 此外，她的教育活动鼓励代表性不足的群体从事科学事业。