Wechselwirkungen von polyphilen Molekülen mit Lipidmonolayern und Lipidvesikeln

多亲分子与脂质单层和脂质囊泡的相互作用

• 批准号: 161163068
• 负责人: Professor Dr. Alfred Blume
• 金额: --
• 依托单位: Arbeitsbereich Physikalische und Theoretische Chemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/161163068?language=en
• 关键词: Wechselwirkungen; von; polyphilen; Molek; len

Goals of this project are investigations and elucidation of the interactions of low molecular weight and polymeric polyphilic molecules in aqueous solution with lipid model membranes and lipid monolayers. Low molecular weight polyphiles will have T- and X-like structures and will be synthesized in the group of C. Tschierske (TP 2) while the polymeric polyphiles will be obtained from the group Kreßler (TP 3). Investigations on the lyotropic behaviour and their aggregation in water will be carried out first. Thereby influence of the chemical structure of the side chains as well as the block sequence will be studied followed by investigations of the surface activity of the compounds at the air-water interface. Lipid model membranes and lipid monolayers will serve as templates for the binding of the polyphiles and resulting structure formation at the interface. The binding strength is due to hydrophobic and hydrophilic interactions with the membranes and will be varied by changing the chemical structure of the polyphiles. The basic methods for studying the aggregation behaviour are light scattering methods, transmission electron microscopy, isothermal titration calorimetry (ITC) for the determination of the critical micellar concentration (cmc), as well as monolayer methods (epi-fluorescence-spectroscopy, infrared-reflection-absorptionspectroscopy (IRRAS) Brewster-angle-microscopy). Studies on the interaction of polyphiles with lipid bilayers and lipid monolayers will be carried out in a parallel fashion. Goal of studies with the low molecular weight polyphiles is to modify the interactions of these molecules and the resulting structure formation and segregation in or at the membranes by changes in the lateral chain structure. For polymeric polyphiles possible chiral recognition between polymers and chiral lipids plays an important addional role. For these studies thermal methods such as ITC and DSC will be used as well as spectroscopic methods such as FT-IR-Spectroscopy on solid supported membranes (FT-IR-ATR) and FT-IRRAS on lipid monolayers. In cooperation with the group Bacia (TP 6) CLSM studies on GUVs will be performed coupled with dye efflux measurements. Similar dye release experiments with small vesicles will also be carried out to elucidate the integrity of the permeation barrier of the membrane after incorporation of polyphiles. In close cooperation with the group Saalwächter (TP 5) additional NMR-experiments will be carried out with the same systems.

该项目的目标是研究和阐明水溶液中低分子量和聚合多亲分子与脂质模型膜和脂质单层的相互作用。低分子量多亲分子将具有 T 和 X 样结构，并将在该组中合成。 C. Tschierske (TP 2) 的研究人员获得了聚合物多亲性物质 (TP 3) 的溶致行为及其聚集研究。首先将研究侧链化学结构以及嵌段序列的影响，然后研究化合物在空气-水界面和脂质单层的表面活性。将作为亲水性物质结合的模板，并在界面处形成结构。结合强度是由于与膜的疏水性和亲水性相互作用而产生的，并且会通过改变亲水性物质的化学结构而改变。聚合行为包括光散射方法、透射电子显微镜、用于测定临界胶束浓度 (cmc) 的等温滴定量热法 (ITC)，以及单层方法（落射荧光光谱法、红外反射吸收光谱法 (IRRAS)、布鲁斯特-多亲性物质与脂质双层和脂质单层相互作用的研究将以并行方式进行。与低分子量亲水性物质的相互作用是通过改变侧链结构来改变这些分子的相互作用以及由此产生的结构形成和膜内或膜上的分离。对于聚合性亲水性物质，聚合物和手性脂质之间可能的手性识别起着重要的附加作用。对于这些研究，将使用 ITC 和 DSC 等热方法以及固体支撑膜上的 FT-IR 光谱 (FT-IR-ATR) 和脂质上的 FT-IRRAS 等光谱方法与 Bacia (TP 6) 小组合作，还将对 GUV 进行 CLSM 研究，并结合染料流出测量进行类似的染料释放实验，以阐明掺入后膜渗透屏障的完整性。与 Saalwächter (TP 5) 小组密切合作，将使用相同的系统进行额外的 NMR 实验。