Wechselwirkungen von polyphilen Molekülen mit Lipidmonolayern und Lipidvesikeln

多亲分子与脂质单层和脂质囊泡的相互作用

• 批准号: 161163068
• 负责人: Professor Dr. Alfred Blume
• 金额: --
• 依托单位: Arbeitsbereich Physikalische und Theoretische Chemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/161163068?language=en
• 关键词: Wechselwirkungen; von; polyphilen; Molek; len

Goals of this project are investigations and elucidation of the interactions of low molecular weight and polymeric polyphilic molecules in aqueous solution with lipid model membranes and lipid monolayers. Low molecular weight polyphiles will have T- and X-like structures and will be synthesized in the group of C. Tschierske (TP 2) while the polymeric polyphiles will be obtained from the group Kreßler (TP 3). Investigations on the lyotropic behaviour and their aggregation in water will be carried out first. Thereby influence of the chemical structure of the side chains as well as the block sequence will be studied followed by investigations of the surface activity of the compounds at the air-water interface. Lipid model membranes and lipid monolayers will serve as templates for the binding of the polyphiles and resulting structure formation at the interface. The binding strength is due to hydrophobic and hydrophilic interactions with the membranes and will be varied by changing the chemical structure of the polyphiles. The basic methods for studying the aggregation behaviour are light scattering methods, transmission electron microscopy, isothermal titration calorimetry (ITC) for the determination of the critical micellar concentration (cmc), as well as monolayer methods (epi-fluorescence-spectroscopy, infrared-reflection-absorptionspectroscopy (IRRAS) Brewster-angle-microscopy). Studies on the interaction of polyphiles with lipid bilayers and lipid monolayers will be carried out in a parallel fashion. Goal of studies with the low molecular weight polyphiles is to modify the interactions of these molecules and the resulting structure formation and segregation in or at the membranes by changes in the lateral chain structure. For polymeric polyphiles possible chiral recognition between polymers and chiral lipids plays an important addional role. For these studies thermal methods such as ITC and DSC will be used as well as spectroscopic methods such as FT-IR-Spectroscopy on solid supported membranes (FT-IR-ATR) and FT-IRRAS on lipid monolayers. In cooperation with the group Bacia (TP 6) CLSM studies on GUVs will be performed coupled with dye efflux measurements. Similar dye release experiments with small vesicles will also be carried out to elucidate the integrity of the permeation barrier of the membrane after incorporation of polyphiles. In close cooperation with the group Saalwächter (TP 5) additional NMR-experiments will be carried out with the same systems.

该项目的目标是用脂质模型膜和脂质单层的水溶液中低分子量和聚合物多态性分子的相互作用的投资和阐明。低分子量的多态料将具有T类和X样结构，并将在C. tschierske组（TP 2）中合成，而聚合物多芯片将从Kreßler组获得（TP 3）。首先将对裂解行为及其在水中的聚集进行调查。因此，将通过空气水界面的化合物表面活性进行投资来研究侧链的化学结构以及块序列的影响。脂质模型机理和脂质单层将用作模板，用于在界面上结合多态料和产生的结构形成。结合强度是由于疏水性和亲水性与机制的相互作用引起的，并且会通过改变多态性的化学结构而变化。研究聚集行为的基本方法是光散射方法，传输电子显微镜，等温滴定热量法（ITC），用于测定关键显微镜（CMC）（CMC）以及单层方法（Epi-Fluorescoppy-encecopicy-spectroscopicy-specopocigy-inffection-reflection-Reflection-Reflection-reflection-absorpections）。关于多态料与脂质双层和脂质单层的相互作用的研究将以平行的方式进行。低分子量多芯片的研究的目标是修改这些分子的相互作用，并通过横向链结构的变化在机制中或在机制中产生的结构形成和隔离。对于聚合物多态子，聚合物和手性脂质之间可能的手性识别起着重要的附加作用。对于这些研究，将使用诸如ITC和DSC之类的热方法以及诸如固体支持机制（FT-IR-ATR）和脂质单层的FT-IR光谱法（FT-IR-光谱法）和FT-IRRAS。将与Babia组（TP 6）CLSM研究对GUV进行研究，并将其与染料外排测量结合。还将使用小型蔬菜进行类似的染料释放实验，以阐明使用多个材料后膜的渗透屏障的完整性。与Saalwächter组密切合作（TP 5）将使用相同的系统进行其他NMR示例。