Collaborative Research: Perception of the Steroid Molting Hormone, 20-Hydroxyecdysone (20-HE) and its Role in Orchestrating Aggressive Interactions in the American Lobster.

合作研究：类固醇蜕皮激素 20-羟基蜕皮激素 (20-HE) 的感知及其在协调美国龙虾攻击性相互作用中的作用。

• 批准号: 0234555
• 负责人: Stuart Cromarty
• 金额: --
• 依托单位: Assumption College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0234555&HistoricalAwards=false
• 关键词: Collaborative; Research; Perception; Steroid; Molting

Perception of the Steroid Molting Hormone, 20-Hydroxyecdysone (20-HE) and its Role in Orchestrating Aggressive Interactions in the American Lobster.The purpose of this research is to identify and analyze the role of steroid hormones in aggressive behavior. The study will investigate how the perception of the steroid molting hormone affects aggression in a model animal, the American lobster. Aggression is a vital component of the survival strategies of all animals and is used in the acquisition of food, mates, and territory. The research will contribute significantly to the understanding of how steroid hormones modulate aggressive behavior. We expect to show that steroids not only alter the internal aggressive state of an animal, but are also used as signaling tools to modify the behavior of an opponent in an aggressive interaction.A primary goal of the project is to give undergraduates an intensive research experience in up-to-date methods of neurobiology. The project is a collaboration between Kass-Simon at the University of Rhode Island, and Cromarty at Assumption College. It will involve URI graduate students, Assumption College undergraduates, and under-represented minority undergraduates from the New England Board of Higher Education's Excellence Through Diversity Initiative Program. The following questions will be addressed:1. Does smelling the steroid molting hormone affect fighting behavior? These are behavioral experiments in which lobsters deprived of their sense of smell fight animals who are exposed to steroids (or placebos). The experiments stem from earlier work showing that injected steroids increase lobster aggressiveness. 2. Are there molt-related differences in the receptor-responses to the molting hormone? These are electrophysiological experiments designed to show how olfactory cells respond electrically to the molting hormone as lobsters go from their hard-shelled (aggressive) to soft-shelled (timid) states. 3. Where are the molting hormone receptors located? These are microscopy experiments, in which fluorescent antibodies are used to locate the steroid receptors on the olfactory nerves. The experiments will augment the electrophysiology experiments.

对类固醇蜕皮激素 20-羟基蜕皮激素 (20-HE) 的认识及其在协调美国龙虾攻击性相互作用中的作用。本研究的目的是识别和分析类固醇激素在攻击性行为中的作用。 这项研究将调查类固醇蜕皮激素的感知如何影响模型动物——美国龙虾的攻击性。攻击性是所有动物生存策略的重要组成部分，用于获取食物、配偶和领地。 这项研究将极大地有助于理解类固醇激素如何调节攻击行为。我们希望证明类固醇不仅可以改变动物的内部攻击状态，而且还可以用作信号工具来改变攻击性互动中对手的行为。该项目的主要目标是为本科生提供深入的研究经验采用最新的神经生物学方法。 该项目是罗德岛大学的 Kass-Simon 和易三仓学院的 Cromarty 之间的合作项目。 它将涉及 URI 研究生、易三仓学院本科生以及来自新英格兰高等教育委员会“多元化卓越计划”的少数族裔本科生。将解决以下问题： 1．闻类固醇蜕皮激素会影响战斗行为吗？ 这些行为实验中，被剥夺嗅觉的龙虾与接触类固醇（或安慰剂）的动物进行战斗。 这些实验源于早期的研究，表明注射类固醇会增加龙虾的攻击性。 2. 受体对蜕皮激素的反应是否存在与蜕皮相关的差异？ 这些电生理学实验旨在展示当龙虾从硬壳（攻击性）状态转变为软壳（胆怯）状态时，嗅觉细胞如何对蜕皮激素做出电反应。 3. 蜕皮激素受体位于哪里？这些是显微镜实验，其中荧光抗体用于定位嗅觉神经上的类固醇受体。这些实验将增强电生理学实验。