Functional Studies of Drosophila Genes with Enriched Expression in the Wing Discovered by Microarray Analyis

通过微阵列分析发现果蝇翅膀中富集表达的基因的功能研究

• 批准号: 0212637
• 负责人: Amanda Simcox
• 金额: $ 39万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0212637&HistoricalAwards=false
• 关键词: Functional; Studies; Drosophila; Genes; Enriched

0212637SimcoxThe Drosophila wing is an important model for analyzing how signaling pathways are coordinated to organize development of a cell field and link patterning with growth. Discovering all the players will be key to a full understanding of the processes involved. Additional genes with wing-enriched expression patterns have been discovered by microarray analysis, here a functional-genomics approach will be taken to probe the roles of these genes in wing development. Oligonucleotide microarrays were used to identify thirty transcripts with four-fold or greater enrichment in the wing versus the body wall. These include genes known to be specifically expressed in the wing (for example, nubbin, Distal-less, knot, and bifid). Here it is proposed to test whether uncharacterized transcripts with similar enrichment have functional roles in wing development and to analyze how they are regulated by major signaling pathways. The first three objectives relate to this goal. In the first, the functional roles of the wing-enriched genes will be investigated by examining phenotypes resulting from ectopic expression and following gene silencing. In the second, the role of the morphogens, Wingless, Hedgehog and Decapentaplegic, in controlling the expression of these novel genes will be assayed. In the third, targets of Epidermal Growth Factor Receptor (EGFR) signaling will be identified by comparing EGFR mutant wings with wild type wings in microarray experiments. The function of novel targets will be investigated. In the fourth objective, a workshop will be offered for high school students to investigate the expression pattern of genes that have the potential to encode posterior-specific transcripts.

0212637SIMCOXTHE果蝇翼是分析信号通路如何协调以组织细胞场的发展并将其与生长联系起来的重要模型。 发现所有玩家将是对所涉及过程的充分理解的关键。 通过微阵列分析发现了具有富含机翼的表达模式的其他基因，这里将采用功能性基因组学方法来探测这些基因在机翼发育中的作用。 寡核苷酸微阵列用于识别三十张转录本在机翼与体壁中具有四倍或更高富集的转录本。 这些包括已知在机翼中特异性表达的基因（例如nubbin，远端，结和分叉）。 在这里，建议测试具有相似富集的未表征的转录本在机翼发育中具有功能作用，并分析如何通过主要信号通路调节它们。 前三个目标与此目标有关。 首先，将通过检查异位表达和基因沉默后引起的表型来研究富含翼的基因的功能作用。 在第二个中，将分析形态，无翅，刺猬和脱牙性的作用，在控制这些新基因的表达中。 在第三次中，将通过在微阵列实验中将EGFR突变翼与野生型机翼进行比较，可以确定表皮生长因子受体（EGFR）信号的靶标。 将研究新目标的功能。 在第四个目标中，将为高中生提供一个研讨会，以研究具有编码后部特异性转录本潜力的基因表达模式。