Construction of BAC Libraries of Metazoans Occupying Key Phylogenetic Positions

系统发育关键位置的后生动物 BAC 文库的构建

• 批准号: 0208138
• 负责人: Linda Holland
• 金额: $ 144.73万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Cooperative Agreement
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0208138&HistoricalAwards=false
• 关键词: Construction; BAC; Libraries; Metazoans; Occupying

Large-insert libraries of genomic DNA are a valuable resource for comparative studies indevelopment evolution and genetics for addressing such fundamental questions in biology ashow genomes have evolved and how evolution of genomes has resulted in evolution of bodyplans. The goal of the proposed research is to construct 11 BAC libraries representative of thebreadth of the Metazoa. The focus is on animals that occupy phylogenetic positions key to anunderstanding of animal evolution. Preference is given to organisms with demonstrated potentialas developmental model organisms. Therefore, 2 deuterostomes have been chosen: lamprey,basal in the craniates and pivotal for understanding the pattern of gene and chromosomalduplications during vertebrate evolution, and the cephalochordate amphioxus, the closest livinginvertebrate relative of the vertebrates. On the basis of molecular phylogenetics, the protostomeshave recently been divided into two groups, the Ecdysozoa and the Lophotrochozoa. However,branching patterns of the phyla within each of these groups are poorly resolved. Becauserepresentatives of the Ecdysozoa, Drosophila and Caenorhabditis have been well-studied, wehave not chosen any Ecdysozoan. For the Lophotrochozoa, which have, in general, received littleattention in regard to genes, development and evolution, seven organisms have been chosen.These include 2 annelids, one of which, the polychaete Platynereis dumerilii, is relatively basalwithin the annelids and the other of which, the leech Helobdella robusta, is more highly derived.Platynereis is rapidly becoming a lophotrochozoan model developmental organism, and whilethe leech is quite derived, it has been the object of developmental genetic studies for some years.Two molluscs are included-- one bivalve, (Spisula solidissima) and one gastropod (Ilyanassaobsoleta). The other Lophotrochozoans include 1 nemertean (Cerebratulus lacteus), 1 sipunculid(Temiste langiformis), and 1 acoel flatworm (Neochildea fusca). The phylogenetic position ofthe acoels is controversial. On the basis of morphology, they were considered basal Bilateria.However, most, but not all, molecular phylogenetic analyses place acoels within the protostomes.To address bilaterian origins, we have chosen a ctenophore (Mnemiopsis leidyi). Finally, toaddress the origin of the metazoa, a sponge, Callyspongia diffusa, a representative of the mostbasal Metazoa, was selected. BAC libraries will be constructed and distributed by P. de Jong(Children's Hospital Research Institute, Oakland), who has a long track-record in construction ofBAC libraries, including making the BAC library for the human genome project. For organismsfor which interest is high to sequence the entire genome, 15X coverage is proposed. For others,10X coverage is proposed.

基因组DNA的大型插入文库是比较研究非洲研究进化和遗传学的宝贵资源，用于解决生物学中的这种基本问题，以及基因组的进化如何导致身体计划的进化。拟议的研究的目的是构建代表Metazoa的Breadth的11个BAC图书馆。重点是占据系统发育位置的动物，这是动物进化的厌食的关键。优先考虑具有证明潜在发展模型生物的生物体。因此，已经选择了2种氘代表：lamp虫，颅底的基础和关键，以理解脊椎动物演化过程中基因和染色体的模式，以及头chalo骨的静脉内，是脊椎动物的最闭合的livinginvertebrate。在分子系统发育学的基础上，原恒星最近分为两组ecdysozoa和lophotrochozoa。但是，这些组内的门的分支模式的解决方案很差。由于经过充分研究了Ecdysozoa，果蝇和Caenorhabditis的表现，因此没有选择任何Ecdysozoan。对于通常在基因，发育和进化方面都接受了少量的酸植物，已经选择了七种生物。其中包括2个Annelids，其中一个，其中一个是plychaete platynereis dumerilii，是相对较大的底层，是rob的insalis and iispler inspler inspler inspl sellery ispl sella，几年来，它迅速成为一种植物学模型的发育生物，尽管水ech是相当得出的，但它一直是发育遗传学研究的对象。其中包括一个软体动物 - 一个小软体动物 - 一个双壳类，（spisula solidissimsima）和一只腹足动物（Ilyananassaobsaobsaobsaobsoleta）。另一个lophotrochozoans包括1个Nemertean（乳酸脑），1个sipunculid（temiste langiboris）和1个Acoel Flatem（Neochildea Fusca）。 Acoels的系统发育位置是有争议的。在形态的基础上，它们被认为是基础双齿。但是，大多数（但不是全部）分子系统发育分析将Acoels放置在原始体内。为了解决双尾巴的起源，我们选择了cte虫（Mnemiopsis leidyi）。最后，选择了蟾蜍，选择了Mostbasal Metazoa的代表Callyspongia diffusa的海绵的起源。 BAC图书馆将由P. de Jong（奥克兰儿童医院研究所）建造和分发，他在BAC图书馆的建设中拥有长期的曲目，包括为人类基因组项目制作BAC图书馆。对于对整个基因组进行测序的兴趣较高的生物体，提出了15倍覆盖范围。对于其他人，提出了10倍的覆盖范围。