Finding the genetic basis of prezygotic isolation barriers in the moth Spodoptera frugiperda

寻找草地贪夜蛾合子前隔离屏障的遗传基础

基本信息

批准号：
155608721
负责人：
Professorin Dr. Astrid T. Groot
金额：
--
依托单位：
Institute for Biodiversity and Ecosystem Dynamics (IBED)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2009
资助国家：
德国
起止时间：
2008-12-31 至 2012-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/155608721?language=en
关键词：
Finding genetic basis prezygotic isolation

项目摘要

The genetic basis of prezygotic isolation barriers is very poorly understood. The noctuid moth Spodoptera frugiperda (Fall armyworm), a pest species occurring sympatrically in the Americas, consists of two host strains, a corn and a rice strain. In this species three prezygotic isolation barriers are found: a) habitat isolation through host plant preference, b) behavioral isolation through differences in sex pheromone composition, and c) behavioral isolation through differential timing of mating activity at night. This proposal aims to find the genetic basis of these three prezygotic isolation barriers. By crossing and backcrossing strains of these two host races, we will conduct quantitative trait locus (QTL) analysis to assess prezygotic traits and location of genes. By phenotyping all traits in the same backcrosses, this project will have a unique opportunity to determine whether the same or different genomic regions underly the different prezygotic isolation barriers. The relative importance of the main QTL of each trait will be assessed experimentally by introgressing the major loci into separate homogeneous genetic backgrounds, and scoring all phenotypic assays with these separate lines. In this way, the effect of each QTL can be specifically measured and possible pleiotropic effects and/or epistatic interactions can be detected. The biological significance and variation of determined prezygotic differences found in the laboratory will be tested in the field at two different sites. (1519).

人们对合子前隔离屏障的遗传基础知之甚少。夜蛾 Spodoptera frugiperda（秋粘虫）是一种在美洲同域发生的害虫，由两种宿主菌株组成：玉米菌株和水稻菌株。在这个物种中，发现了三个合子前的隔离屏障：a）通过寄主植物偏好进行栖息地隔离，b）通过性信息素成分差异进行行为隔离，以及c）通过夜间交配活动的不同时间进行行为隔离。该提案旨在找到这三种合子前隔离障碍的遗传基础。通过这两个宿主种族的杂交和回交，我们将进行数量性状基因座（QTL）分析，以评估合子前性状和基因位置。通过对同一回交中的所有性状进行表型分析，该项目将有一个独特的机会来确定相同或不同的基因组区域是否是不同的合子前隔离屏障的基础。每个性状的主要QTL的相对重要性将通过将主要基因座渗入单独的同质遗传背景并用这些单独的品系对所有表型测定进行评分来通过实验评估。通过这种方式，可以具体测量每个QTL的效应，并且可以检测可能的多效性效应和/或上位相互作用。实验室中发现的确定的合子前差异的生物学意义和变化将在两个不同地点进行现场测试。（1519）。