CAREER: Integrated-Optic Nanoparticle Biosensor Arrays

职业：集成光学纳米颗粒生物传感器阵列

• 批准号: 0134548
• 负责人: Steven Blair
• 金额: $ 37.5万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-15 至 2008-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0134548&HistoricalAwards=false
• 关键词: CAREER; Integrated; Optic; Nanoparticle; Biosensor

Research world-wide on biosensing is motivated by numerous applications in environmental and food testing and clinical diagnostics, for example. However, the important problem of detecting in parallel a large number of molecular species from the very small samples typical of most collection procedures remains an elusive goal. This CAREER research plan focuses on solving this problem by merging the science of nanophotonics with waveguide biosensors and microfluidics for the development of a new class of molecular detection array.The immobilization of metallic nanoparticles onto discrete zones of an optical waveguide surface makes the parallel detection of a large number of molecular species feasible. In each zone, capture molecules tethered to the nanoparticles preferentially bind to a particular molecular species through an affinity interaction. Strong localization of light about each nanoparticle allows for dramatic improvement in optical signal transduction, thereby facilitating the detection of small numbers of molecules bound within each zone.Microfluidics will be used to deliver small sample volumes to each sensing zone and passive mixing structures will be studied in order to increase the molecular binding probability within each zone.The education plan focuses on the creation of a summer optics workshop for secondary school physics and science teachers. As more demands are placed on teachers, and as technology continues to advance at a rapid pace, teachers need a way in which to further their knowledge of science and hands-on teaching methods. Detailed lesson plans and laboratory exercises will be developed for deployment in the classroom, with the goal of improving student understanding of and instruction in optics and the sciences, and encouraging students to pursue careers in engineering and science. Participation of teachers from Hispanic and Native American schools will be strongly encouraged.

例如，在世界范围内进行生物传感的研究是由在环境和食物测试以及临床诊断方面进行的许多应用。 但是，从大多数收集程序的典型样本中检测到大量分子物种的重要问题仍然是一个难以捉摸的目标。该职业研究计划着重于通过将纳米素化学的科学与波导生物传感器和微富集学合并，以开发新的分子检测阵列，将金属纳米颗粒固定在光学波形表面的离散区域上，使得并行检测可行的大量分子物种。 在每个区域中，捕获分子束缚在纳米颗粒上，优先通过亲和力相互作用与特定分子物种结合。 在每个纳米颗粒上的光的强烈定位允许光学信号转导的显着改善，从而促进检测每个区域内结合的少量分子。Microfluidics将用于向每个感应区域传递少量样品体积，并研究被无源混合结构。为了增加每个区域内的分子结合概率。教育计划着重于为中学物理和科学教师创建夏季光学研讨会。 随着对教师的越来越多的要求，随着技术继续以快速的速度发展，教师需要一种方法来进一步了解他们对科学和动手教学方法的了解。 详细的课程计划和实验室练习将在课堂上开发，以提高学生对光学和科学的教学的理解和教学，并鼓励学生从事工程和科学的职业。 来自西班牙裔和美国原住民学校的教师的参与将受到强烈的鼓励。