CAREER: The Behavior of Proteins at the Solid-Liquid Interface Monitored in situ with Infrared-Visible Sum Frequency Generation: Investigating the Role of Interfacial Water

职业：利用红外-可见光和频生成原位监测固-液界面蛋白质的行为：研究界面水的作用

• 批准号: 0094332
• 负责人: Paul Cremer
• 金额: $ 48.2万
• 依托单位: Texas A&M Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0094332&HistoricalAwards=false
• 关键词: CAREER; Behavior; Proteins; Solid; Liquid

This CAREER project addresses the molecular level structure of water at the liquid solid interface in the presence of adsorbed protein molecules. Professor Paul Cremer and his coworkers at Texas A&M, with the support of the Analytical and Surface Chemistry Program, will examine the interactions of water, protein molecules, and solid silica surfaces. An understanding of these types of systems is important to a variety of applications, including biocompatibility, biofouling in the food and pharmaceutical industries, and in the development of biosensors and catalysts. Infrared-visible sum frequency generation methods will be used to probe the structure of substrate associated water in the presence of adsorbed protein. Time resolved studies will be implemented to follow structural changes upon adsorption of protein molecules at the solid surface. Educational aspects of this project include the development of a bio-physical chemistry course for upper level undergraduates, and the implementation of undergraduate research projects in the field of protein surface interactions. The interaction of protein molecules with surfaces is important in the development of biosensors, in enhancing biocompatibility for medical implants, and for understanding the process of biofouling in food and pharmaceutical process machinery. The structure of adsorbed water in these systems provides the detailed information that can be used to design surfaces with desired protein adsorption properties. This CAREER project uses state of the art surface spectroscopic methods to probe this structural question. Integration of this basic research with educational goals in the training of bio-physical scientists and in the development of course materials in this area is the focus of the educational aspect of this project.

该职业项目研究了存在吸附蛋白质分子的情况下液固界面处水的分子水平结构。 德克萨斯农工大学的 Paul Cremer 教授和他的同事在分析和表面化学项目的支持下，将研究水、蛋白质分子和固体二氧化硅表面的相互作用。 了解这些类型的系统对于各种应用都很重要，包括生物相容性、食品和制药行业的生物污垢以及生物传感器和催化剂的开发。 红外-可见光和频产生方法将用于探测存在吸附蛋白质的底物相关水的结构。 将实施时间分辨研究，以跟踪蛋白质分子在固体表面吸附时的结构变化。 该项目的教育方面包括为高年级本科生开发生物物理化学课程，以及实施蛋白质表面相互作用领域的本科生研究项目。蛋白质分子与表面的相互作用对于生物传感器的开发、增强医疗植入物的生物相容性以及了解食品和制药加工机械中的生物污垢过程非常重要。 这些系统中吸附水的结构提供了详细信息，可用于设计具有所需蛋白质吸附特性的表面。 这个职业项目使用最先进的表面光谱方法来探讨这个结构问题。 将这项基础研究与生物物理科学家培训和该领域课程材料开发的教育目标相结合是该项目教育方面的重点。