RUI: Molecular Systematics of the Conifers: Chloroplast DNA Structural Evidence

RUI：针叶树的分子系统学：叶绿体 DNA 结构证据

• 批准号: 0075700
• 负责人: Linda Raubeson
• 金额: $ 20万
• 依托单位: Central Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0075700&HistoricalAwards=false
• 关键词: RUI; Molecular; Systematics; Conifers; Chloroplast

0075700Raubeson The cone-bearing plants or conifers, including pines, firs, junipers, cypresses, and relatives, number in the several hundred species of living plants, disposed in 60 or so genera, and are the surviving members of an evolutionary lineage that dominated terrestrial environments in the Mesozoic. Genealogical relationships among these surviving groups are not yet firmly established, although gene sequence data are accumulating for many representative taxa. Dr. Linda Raubeson is contributing new structural genomic data to help answer questions about the phylogenetic relationships of conifers. In particular, she is studying the content and order of genes in the chloroplast genome of a sample of 20 or so genera of conifers, and including the likely related ginkgo and gnetophytes. Fine-scale restriction-enzyme mapping of mutations along the chloroplast genome will reveal changes in gene order (inversions or translocations) and gene losses or duplications, some of which have already been characterized in her laboratory. Most of these structural mutations are of rare occurrence, and are likely to mark groups of phylogenetically related genera, thus providing new evidence for taxonomic and classification studies. The chloroplast genome maps, in turn, will aid more detailed studies of species-level relationships in taxonomically problematic groups, either by gene sequencing or yet finer scale restriction-enzyme mapping. Throughout the project, as in past work by Dr. Raubeson, undergraduate students will participate in discrete activities targeted to provide them training in modern molecular phylogenetic methods and experience in the design and execution of a research activity.

0075700Raubeson 球果植物或针叶树，包括松树、冷杉、杜松、柏树和近缘植物，有数百种活植物，分布在 60 个左右属中，是主导陆地的进化谱系的幸存成员中生代的环境。尽管许多代表性分类单元的基因序列数据正在积累，但这些幸存群体之间的谱系关系尚未牢固建立。 Linda Raubeson 博士正在贡献新的结构基因组数据，以帮助回答有关针叶树系统发育关系的问题。她正在研究大约 20 个针叶树属样本的叶绿体基因组中基因的内容和顺序，其中包括可能相关的银杏和轮叶植物。叶绿体基因组突变的精细限制性酶图谱将揭示基因顺序的变化（倒位或易位）以及基因丢失或重复，其中一些已经在她的实验室中得到了表征。这些结构突变大多数很少发生，并且可能标记系统发育相关属的群体，从而为分类学和分类研究提供新的证据。反过来，叶绿体基因组图谱将有助于通过基因测序或更精细的限制性酶图谱对分类学问题群体中的物种水平关系进行更详细的研究。在整个项目中，与 Raubeson 博士过去的工作一样，本科生将参加离散的活动，旨在为他们提供现代分子系统发育方法的培训以及研究活动设计和执行的经验。