RUI: Molecular Systematics of the Conifers: Chloroplast DNA Structural Evidence

RUI：针叶树的分子系统学：叶绿体 DNA 结构证据

• 批准号: 0075700
• 负责人: Linda Raubeson
• 金额: $ 20万
• 依托单位: Central Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0075700&HistoricalAwards=false
• 关键词: RUI; Molecular; Systematics; Conifers; Chloroplast

0075700Raubeson The cone-bearing plants or conifers, including pines, firs, junipers, cypresses, and relatives, number in the several hundred species of living plants, disposed in 60 or so genera, and are the surviving members of an evolutionary lineage that dominated terrestrial environments in the Mesozoic. Genealogical relationships among these surviving groups are not yet firmly established, although gene sequence data are accumulating for many representative taxa. Dr. Linda Raubeson is contributing new structural genomic data to help answer questions about the phylogenetic relationships of conifers. In particular, she is studying the content and order of genes in the chloroplast genome of a sample of 20 or so genera of conifers, and including the likely related ginkgo and gnetophytes. Fine-scale restriction-enzyme mapping of mutations along the chloroplast genome will reveal changes in gene order (inversions or translocations) and gene losses or duplications, some of which have already been characterized in her laboratory. Most of these structural mutations are of rare occurrence, and are likely to mark groups of phylogenetically related genera, thus providing new evidence for taxonomic and classification studies. The chloroplast genome maps, in turn, will aid more detailed studies of species-level relationships in taxonomically problematic groups, either by gene sequencing or yet finer scale restriction-enzyme mapping. Throughout the project, as in past work by Dr. Raubeson, undergraduate students will participate in discrete activities targeted to provide them training in modern molecular phylogenetic methods and experience in the design and execution of a research activity.

0075700000000700RAUBESON带有锥形植物或针叶树，包括松树，冷杉，杜松，柏树和亲戚，数百种生物植物中的数字，在60个左右的属中处置，并且是中学领域中占主导地位环境的尚存成员。尽管基因序列数据积累了许多代表性分类单元，但这些幸存群体之间的家谱关系尚未牢固确定。 Linda Raubeson博士正在贡献新的结构基因组数据，以帮助回答有关针叶树系统发育关系的问题。特别是，她正在研究20种左右的针叶树样品中叶绿体基因组中基因的含量和顺序，包括可能相关的银杏和gnetophytes。沿叶绿体基因组突变的细小限制 - 酶映射将揭示基因顺序（反转或易位）和基因丢失或重复的变化，其中一些已经在她的实验室中表征了。这些结构突变大多数很少发生，并且很可能标志着系统发育相关的属，因此为分类学和分类研究提供了新的证据。反过来，叶绿体基因组图将有助于对分类学有问题的组中物种水平关系的更详细的研究，无论是通过基因测序还是更细的量表限制 - 酶映射。在整个项目中，就像劳伯森博士过去的工作一样，本科生将参加针对现代分子系统发育方法培训的离散活动，并在设计和执行研究活动方面的经验。