Collaborative Research: Analysis of Metal Chelators in Marine Systems by HPLC with Derivatization

合作研究：通过衍生化 HPLC 分析海洋系统中的金属螯合剂

• 批准号: 0002677
• 负责人: Beth Ahner
• 金额: --
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0002677&HistoricalAwards=false
• 关键词: Collaborative; Research; Analysis; Metal; Chelators

ABSTRACTOCE-0002677Almost all biologically active trace metal cations in seawater are complexed by organic ligands, and complexation is believed to exert an important influence on the geochemistry and biological availability of these elements, many of which are critical micronutrients. However, there is little, if any, information on the structures of these compounds, or any definitive information on sources and sinks, though biological sources have been inferred on the basis of their distributions and binding characteristics. Some researchers have been successful in isolating and identifying chelators produced by cultures of marine bacteria and phytoplankton, namely Fe siderophores and phytochelatins that strongly complex Cu. In this study, the PIs will develop sensitive assays for Fe and Cu chelators using liquid chromatography coupled to a selection of highly promising derivatization schemes to measure these compounds directly in seawater at nanomolar levels. This is a novel approach, based on the observation that many of these ligands contain functional groups that form analytically useful adducts with derivatizing agents that can be separated from other materials. The objective of the research is to determine if these compounds are important ligands in seawater, and the assays will serve as a basis for modified protocols to look for structurally similar compounds. The long term goals of the project is to develop useful analytical protocols that will be used widely in the field to answer questions about the factors that control metal complexation in seawater, and their relationship to ecosystem-scale processes.

Abstractoce-0002677最多的所有生物活性痕量金属阳离子都被有机配体络合，并且络合物被认为对这些元素的地球化学和生物学可用性产生重要影响，其中许多元素是关键的微量营养素。但是，尽管已经根据其分布和结合特征推断出了有关这些化合物的结构的信息（如果有的话）或有关来源和水槽的任何确定信息。一些研究人员已经成功地隔离和鉴定由海洋细菌和浮游植物培养物所产生的螯合剂，即强烈复杂的Cu的Fe sideophores和phytochelatins。在这项研究中，PIS将使用液相色谱与选择高度有前途的衍生化方案的选择，对Fe和Cu螯合剂进行敏感测定，以直接在纳摩尔水平的海水中测量这些化合物。这是一种新的方法，基于这样的观察结果，即许多这些配体都包含具有分析有用的加合物的官能团，并具有可以与其他材料分离的衍生剂。该研究的目的是确定这些化合物是否是海水中的重要配体，并且该测定将作为修改方案的基础，以寻找结构相似的化合物。该项目的长期目标是制定有用的分析方案，这些方案将在现场广泛使用，以回答有关控制海水中金属络合的因素及其与生态系统规模过程的关系。