Diffusion and Binding in Polymerized Bicontinuous Cubic Phases

聚合双连续立方相中的扩散和结合

基本信息

批准号：
9816826
负责人：
Neal Armstrong
金额：
$ 14.5万
依托单位：
University of Arizona
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
1999
资助国家：
美国
起止时间：
1999-04-01 至 2001-03-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9816826&HistoricalAwards=false
关键词：
Diffusion Binding Polymerized Bicontinuous Cubic

项目摘要

ABSTRACTCTS-9816826David O'BrienU. of ArizonaNatural and synthetic polar lipids can form a variety of hydrated nonlamellar phases, including the inverted hexagon (HII) and inverted bicontinuous cubic (QII) phases. Lipids that readily form one or more of these nonlamellar phases are sometimes termed polymorphic lipids. Appropriately designed polymerizable polymorphic lipids can be successfully used to form either of these nonlamellar phases and stabilize them via crosslinking polymerization. The bicontinuous cubic morphologies consist of curved lipid bilayers that separate interpentrating water channels. The principal investigator's has introduced a successful strategy for the polymerization of these nonlamellar phases, that retains their morphology and stabilizes the assembly in a manner that enhances both the temperature range and resistance to organic solvents. Thus the lipid domains can be converted to poly(lipid) with a wide range of polymer properties, while leaving the water domains unperturbed for further elaboration. The interpentrating networks of the polymerized QII phases suggests potential applications of these organic zeolites, which take full advantage of the lipid membrane surface for the localization of reagents, the immobilization of antibody fragments or abenzymes, or the incorporation of proteins for chemcial or biological conversions, diagnostic, and/or separation applications. The objective of this proposal is to characterize the effect of molecular size on the diffusion of water-soluble macromolecules within the water channels of polymerized QII phases. Macromolecular diffusion will be experimentally determined using pulsed field gradient NMR techniques. The materials for the formation and stabilization of QII phases will come from a family of readily synthesized polymerizable lipids. This is a terminal grant for one year.

Abstractcts-9816826David O'Brienu。亚利桑那州天然和合成极性脂质可以形成各种水合的非层相，包括倒置的六边形（HII）和倒双偶性立方体（QII）相。容易形成这些非层流中之一的脂质有时被称为多态性脂质。可以成功地使用适当设计的多态性脂质来形成这些非层流中的任何一个，并通过交联聚合稳定它们。双连续的立方形态由弯曲的脂质双层组成，这些双层分离嵌入式水通道。首席研究者已经引入了这些非层次相的聚合策略，该策略保留了它们的形态并以增强温度范围和对有机溶剂的抗性的方式稳定组装。因此，可以将脂质结构域转化为具有多种聚合物特性的聚（脂质），同时使水域不受干扰以进一步阐述。聚合QII阶段的插入网络表明了这些有机沸石的潜在应用，它们可以充分利用脂质膜表面来定位试剂的定位，抗体片段或依次的固定化，或抗化学或生物学对转换的蛋白质的掺入蛋白质，并应用蛋白质。该建议的目的是表征分子大小对聚合QII阶段水通道中水溶性大分子扩散的影响。大分子扩散将使用脉冲场梯度NMR技术实验确定。 QII相的形成和稳定的材料将来自一个容易合成的可聚合脂质的家族。这是一年的终端赠款。